Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jan 17;25(1):72.
doi: 10.1186/s12864-024-09973-x.

Identification of transient receptor potential channel genes from the swimming crab, Portunus Trituberculatus, and their expression profiles under acute temperature stress

Yichen Qian  1 , Qiaoling Yu  1 , Jun Zhang  1 , Yaoyao Han  1 , Xi Xie  2 , Dongfa Zhu  3
Affiliations

Identification of transient receptor potential channel genes from the swimming crab, Portunus Trituberculatus, and their expression profiles under acute temperature stress

Yichen Qian et al. BMC Genomics. .

Abstract

Background: Temperature is an important environment factor that is critical to the survival and growth of crustaceans. However, the mechanisms by which crustaceans detect changes in temperature are still unclear. The transient receptor potential (TRP) channels are non-selective cation channels well known for properties in temperature sensation. However, comprehensive understandings on TRP channels as well as their temperature sensing functions are still lacking in crustaceans.

Results: In this study, a total of 26 TRP genes were identified in the swimming crab, Portunus trituberculatus, which can be classified into TRPA, TRPC, TRPP, TRPM, TRPML, TRPN and TRPV. Tissue expression analysis revealed a wide distribution of these TRP genes in P. trituberculatus, and antennules, neural tissues, and ovaries were the most commonly expressed tissues. To investigate the responsiveness of TRP genes to the temperature change, 18 TRPs were selected to detect their expression after high and low temperature stress. The results showed that 12 TRPs showed induced gene expression in both high and low temperature groups, while 3 were down-regulated in the low temperature group, and 3 showed no change in expression in either group.

Conclusions: This study characterized the TRP family genes in P. trituberculatus, and explored their involvement in response to temperature stress. Our results will enhance overall understanding of crustacean TRP channels and their possible functions.

Keywords: Expression analysis; Molecular characterization; Portunus Trituberculatus; Temperature stress; Transient receptor potential channels.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Distribution of TRP genes in chromosome of P. trituberculatus. Twenty-six TRP genes were mapped on the sixteen P. trituberculatus chromosomes. The scale on the left is in million bases (Mb). Chromosome numbers are indicated at the left of each vertical bar
Fig. 2
Fig. 2
Maximum likelihood phylogenetic tree of TRP channels. Ptri, Portunus trituberculatus; Parg, Panulirus argus; Csap, Callinectes sapidus; Pcla, Procambarus clarkii; Dpul, Daphnia pulex; Dmag, Daphnia magna; Dmel, Drosophila melanogaster; Bmor, Bombyx mori; Tcas, Tribolium castaneum. The species sequence accession numbers are listed in Additional File 1. Various subfamilies of TRP channels are indicated by different colors: TRPA subfamily (red), TRPC (green), TRPN (purple), TRPM (pale orange), TRPML (yellow), TRPP (grey) and TRPV (blue). P. trituberculatus has several homologues to each subfamily of TRP channels
Fig. 3
Fig. 3
The domain organization of TRPC, TRPN, TRPM, and TRPP subfamilies. Schematic diagrams show structures of TRP channels, including transmembrane domains, ankyrin repeats, GPS, TRP domain, PKD-repeats, and LH2. Predicted TRP domain amino acid sequence of TRPL, TRPC-1, TRPC-2, TRPgamma, TRPM, and NompC have been aligned using Clustal X, shown conserved sequence motifs by Jalview 2.11.2
Fig. 4
Fig. 4
Tissue distribution of P. trituberculatus TRP transcripts. Twenty-two TRP transcripts were detected in the fifteen tissues from P. trituberculatus. M, DNA Marker; An1, Antenna 1; An2, Antenna 2; AnG, antennal gland; Br, brain; TG, thoracic ganglion; Ht, heart; Gi, gill; Hp, hepatopancreas; Ms, muscle; Es, eyestalk; Ep, epidermis; In, intestine; Ov, ovary; YO, Y-organ; Te, testis; N, negative control (representing no template in PCR). β-actin was used as the reference gene
Fig. 5
Fig. 5
Relative expression of TRP genes under low-temperature stress in P. trituberculatus. Data are presented as mean ± SD (n = 3). Significant differences among groups are indicated by different letter labels (one-way ANOVA, followed by post hoc Tukey’s multiple-group comparison, P < 0.05)
Fig. 6
Fig. 6
Relative expression of TRP genes under high-temperature stress in P. trituberculatus. Data are presented as mean ± SD (n = 3). Significant differences among groups are indicated by different letter labels (one-way ANOVA, followed by post hoc Tukey’s multiple-group comparison, P < 0.05).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Azra MN, Chen JC, Ikhwanuddin M, Abol-Munafi AB. Thermal tolerance and locomotor activity of blue swimmer crab Portunus pelagicus instar reared at different temperatures. J Therm Biol. 2018;74:234–40. doi: 10.1016/j.jtherbio.2018.04.002. - DOI - PubMed
    1. Azra MN, Chen J-C, Hsu T-H, Ikhwanuddin M, Abol-Munafi AB. Growth, molting duration and carapace hardness of blue swimming crab, Portunus pelagicus, instars at different water temperatures. Aquacult Rep. 2019;15.
    1. Yuan Q, Wang Q, Zhang T, Li Z, Liu J. Effects of water temperature on growth, feeding and molting of juvenile Chinese mitten crab Eriocheir sinensis. Aquaculture. 2017;468:169–74. doi: 10.1016/j.aquaculture.2016.10.007. - DOI
    1. Syafaat MN, Gunarto, Sulaeman H, Ma H, Ikhwanuddin M. Effects of different feeding regimes on larvae and crablets of purple mud crab, Scylla tranquebarica (Fabricius, 1798). Aquacult Rep. 2019;15.
    1. Quinn BK. Threshold temperatures for performance and survival of American lobster larvae: a review of current knowledge and implications to modeling impacts of climate change. Fish Res. 2017;186:383–96. doi: 10.1016/j.fishres.2016.09.022. - DOI

Substances

LinkOut - more resources