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. 2024 Jan 2;14(2):995-1008.
doi: 10.1039/d3ra07078f.

Design, synthesis, pharmacological evaluation, and in silico studies of the activity of novel spiro pyrrolo[3,4- d]pyrimidine derivatives

Abdullah Y A Alzahrani  1 , Wesam S Shehab  2 , Asmaa H Amer  2 , Mohamed G Assy  2 , Samar M Mouneir  3 , Maged A Aziz  2 , Atef M Abdel Hamid  2
Affiliations

Design, synthesis, pharmacological evaluation, and in silico studies of the activity of novel spiro pyrrolo[3,4- d]pyrimidine derivatives

Abdullah Y A Alzahrani et al. RSC Adv. .

Erratum in

Abstract

In the present study, spiro compounds are shown to have distinctive characteristics because of their interesting conformations and their structural impacts on biological systems. A new family of functionalized spiro pyrrolo[3,4-d]pyrimidines is prepared via the one-pot condensation reaction of amino cyclohexane derivatives with benzaldehyde to prepare fused azaspiroundecanedione and azaspirodecenone/thione derivatives. A series of synthesized spiro compounds were scanned against DPPH and evaluated for their ability to inhibit COX-1 and COX-2. All compounds exhibit significant antiinflammatory activity, and they inhibited both COX-1 and COX-2 enzymes with a selectivity index higher than celecoxib as a reference drug. The most powerful and selective COX-2 inhibitor compounds were 11 and 6, with selectivity indices of 175 and 129.21 in comparison to 31.52 of the standard celecoxib. However, candidate 14 showed a very promising antiinflammatory activity with an IC50 of 6.00, while celecoxib had an IC50 of 14.50. Our findings are promising in the area of medicinal chemistry for further optimization of the newly designed and synthesized compounds regarding the discussed structure-activity relationship study (SAR), in order to obtain a superior antioxidant lead compound in the near future. All chemical structures of the novel synthesized candidates were unequivocally elucidated and confirmed utilizing spectroscopic and elemental investigations.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Scheme 1
Scheme 1. Synthetic route of spiro azines 4 and 6.
Scheme 2
Scheme 2. Synthesis of polycyclic azine 11.
Scheme 3
Scheme 3. Synthesis of pyrimidine and pyridine derivatives 14 and 17.
See this image and copyright information in PMC

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