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. 2023 Dec 14;8(51):48689-48703.
doi: 10.1021/acsomega.3c05041. eCollection 2023 Dec 26.

Characterization of Proteins Extracted from Ulva sp., Padina sp., and Laurencia sp. Macroalgae Using Green Technology: Effect of In Vitro Digestion on Antioxidant and ACE-I Inhibitory Activity

Eda Şensu  1   2 , Eda Nur Ayar  1 , Emine Şükran Okudan  3 , Beraat Özçelik  1   4 , Aysun Yücetepe  5
Affiliations

Characterization of Proteins Extracted from Ulva sp., Padina sp., and Laurencia sp. Macroalgae Using Green Technology: Effect of In Vitro Digestion on Antioxidant and ACE-I Inhibitory Activity

Eda Şensu et al. ACS Omega. .

Erratum in

Abstract

Macroalgal proteins were extracted from Ulva rigida (URPE) (green), Padina pavonica (PPPE) (brown), and Laurencia obtusa (LOPE) (red) using ultrasound-assisted enzymatic extraction, which is one of the green extraction technologies. Techno-functional, characteristic, and digestibility properties, and biological activities including antioxidant (AOA) and angiotensin-I converting enzyme (ACE-I) inhibitory activities were also investigated. According to the results, the extraction yield (EY) (94.74%) was detected in the extraction of L. obtusa, followed by U. rigida and P. pavonica. PPPE showed the highest ACE-I inhibitory activity before in vitro digestion. In contrast to PPPE, LOPE (20.90 ± 0.00%) and URPE (20.20 ± 0.00%) showed higher ACE-I inhibitory activity after in vitro digestion. The highest total phenolic content (TPC) (77.86 ± 1.00 mg GAE/g) was determined in LOPE. On the other hand, the highest AOACUPRAC (74.69 ± 1.78 mg TE/g) and AOAABTS (251.29 ± 5.0 mg TE/g) were detected in PPPE. After in vitro digestion, LOPE had the highest TPC (22.11 ± 2.18 mg GAE/g), AOACUPRAC (8.41 ± 0.06 mg TE/g), and AOAABTS (88.32 ± 0.65 mg TE/g) (p < 0.05). In vitro protein digestibility of three macroalgal protein extracts ranged from 84.35 ± 2.01% to 94.09 ± 0.00% (p < 0.05). Three macroalgae showed high oil holding capacity (OHC), especially PPPE (410.13 ± 16.37%) (p < 0.05), but they showed minimum foaming and emulsifying properties. The quality of the extracted macroalgal proteins was assessed using FTIR, SDS-PAGE, and DSC analyses. According to our findings, the method applied for macroalgal protein extraction could have a potential the promise of ultrasonication application as an environmentally friendly technology for food industry. Moreover, URPE, PPPE, and LOPE from sustainable sources may be attractive in terms of nourishment for people because of their digestibility, antioxidant properties, and ACE-I inhibitory activities.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Effect of pH on net surface charge (ζ potential) and solubility of protein extract from (A) U. rigida, (B) L. obtusa, and (C) P. pavonica. Results are given as mean ± standard deviation.
Figure 2
Figure 2
FTIR spectrum of P. pavonica, U. Rigida, and L. obtusa protein extract.
Figure 3
Figure 3
Protein bands by SDS-PAGE of L. obtusa, U. Rigida, and P. pavonica protein extract.
Figure 4
Figure 4
Thermal properties by DSC of P. pavonica, U. Rigida, and L. obtusa protein extract.
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