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. 2023 Dec 1;31(4):539-552.
doi: 10.53854/liim-3104-13. eCollection 2023.

Impact of Nested Multiplex Polymerase Chain Reaction Assay in the management of pediatric patients with acute respiratory tract infections: a single center experience

Basma Sherif  1 , Heba M Hamza  2 , Hoda Ezz Elarab Abdelwahab  1
Affiliations

Impact of Nested Multiplex Polymerase Chain Reaction Assay in the management of pediatric patients with acute respiratory tract infections: a single center experience

Basma Sherif et al. Infez Med. .

Abstract

Purpose: Acute lower respiratory infection (ALRI) remains a global public health problem among children. Distinguishing the etiology of ALRI is challenging and rapid pathogen identification is critical for optimizing the diagnosis and treatment of infectious diseases. Multiplex polymerase chain reaction (PCR) is sensitive, simple, and rapid. Our objective was to evaluate the diagnostic yield and prognostic significance of the FilmArray test for identification of pathogens in pediatric patients with ALRI at a tertiary care center.

Methods: A prospective observational cross-sectional study involved 230 pediatric patients presented with acute lower respiratory tract (LRT) symptoms, for whom conventional bacterial culture and FilmArray testing was ordered to aid in the proper diagnosis of the implicated respiratory pathogens.

Results: FilmArray Respiratory panel (FARP) was positive in 201 patients (87.4%). The most common detected pathogens were Respiratory syncytial virus (RSV), Human Rhinovirus/Enterovirus, Parainfluenza, Influenza A, and Klebsiella pneumoniae; 45 (19.6%), 38 (16.5%), 11 (4.8%), 8 (3.5%) and 6 (2.6%) respectively. FilmArray enabled a change in antimicrobial therapy in 168 (73%) of the patients.

Conclusions: FilmArray enables to improve etiological diagnosis of ALRI and optimize the antimicrobial use of drugs in critical care pediatric patients. Clinical correlation is essential to interpret multiple pathogens and resistance genes.

Keywords: FilmArray; PCR; Point of care testing; Syndromic testing; pediatric respiratory infections; stewardship.

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Conflict of interest statement

Conflict of interest: The authors have no relevant financial or non-financial interests to disclose.

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