Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jul 1;237(1):111-6.
doi: 10.1042/bj2370111.

The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labelling of a component polypeptide of the oxidase

A R Cross, O T Jones

The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labelling of a component polypeptide of the oxidase

A R Cross et al. Biochem J. .

Abstract

NADPH-dependent superoxide production by the solubilized oxidase of neutrophils was inhibited 36% by diphenylene iodonium at a 1:1 stoichiometry with the enzyme flavoprotein content. Addition of diphenylene iodonium strongly inhibited the NADPH-dependent reduction of both FAD and cytochrome b-245 in steady-state kinetic experiments. Incubation of solubilized enzyme with diphenylene [125I]iodonium resulted in the specific labelling of a polypeptide of Mr 45,000. In the presence of NADPH the amount of label incorporated into the polypeptide was reduced. There was no difference in labelling between enzyme prepared from stimulated or unstimulated cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Br J Exp Pathol. 1964 Oct;45:548-59 - PubMed
    1. Biochem J. 1977 Jun 1;163(3):605-15 - PubMed
    1. N Engl J Med. 1978 Mar 23;298(12):659-68 - PubMed
    1. Biochem Soc Trans. 1979 Feb;7(1):103-6 - PubMed

Publication types