Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Mar;36(9):e2307678.
doi: 10.1002/adma.202307678. Epub 2023 Dec 6.

Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery

Affiliations
Review

Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery

Adriana Mulero-Russe et al. Adv Mater. 2024 Mar.

Abstract

Human intestinal organoids (HIOs) derived from pluripotent stem cells or adult stem cell biopsies represent a powerful platform to study human development, drug testing, and disease modeling in vitro, and serve as a cell source for tissue regeneration and therapeutic advances in vivo. Synthetic hydrogels can be engineered to serve as analogs of the extracellular matrix to support HIO growth and differentiation. These hydrogels allow for tuning the mechanical and biochemical properties of the matrix, offering an advantage over biologically derived hydrogels such as Matrigel. Human intestinal organoids have been used for repopulating transplantable intestinal grafts and for in vivo delivery to an injured intestinal site. The use of synthetic hydrogels for in vitro culture and for in vivo delivery is expected to significantly increase the relevance of human intestinal organoids for drug screening, disease modeling, and therapeutic applications.

Keywords: enteroids; human intestinal organoids; hydrogel; poly(ethylene glycol); stem cells.

PubMed Disclaimer

Similar articles

References

    1. Gunther C, Winner B, Neurath MF & Stappenbeck TS Organoids in gastrointestinal diseases: from experimental models to clinical translation. Gut 71, 1892–1908, doi:10.1136/gutjnl-2021-326560 (2022). - DOI - PMC - PubMed
    1. Taelman J, Diaz M & Guiu J Human Intestinal Organoids: Promise and Challenge. Front Cell Dev Biol 10, 854740, doi:10.3389/fcell.2022.854740 (2022). - DOI - PMC - PubMed
    1. Spence JR Taming the Wild West of Organoids, Enteroids, and Mini-Guts. Cell Mol Gastroenterol Hepatol 5, 159–160, doi:10.1016/j.jcmgh.2017.11.003 (2018). - DOI - PMC - PubMed
    1. Hautefort I, Poletti M, Papp D & Korcsmaros T Everything You Always Wanted to Know About Organoid-Based Models (and Never Dared to Ask). Cell Mol Gastroenterol Hepatol 14, 311–331, doi:10.1016/j.jcmgh.2022.04.012 (2022). - DOI - PMC - PubMed
    1. Yui S et al. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5(+) stem cell. Nat Med 18, 618–623, doi:10.1038/nm.2695 (2012). - DOI - PubMed

LinkOut - more resources