Supplementation with the Probiotic Strains Bifidobacterium longum and Lactiplantibacillus rhamnosus Alleviates Glucose Intolerance by Restoring the IL-22 Response and Pancreatic Beta Cell Dysfunction in Type 2 Diabetic Mice

doi:10.1007/s12602-023-10156-5

. 2023 Oct 7.

doi: 10.1007/s12602-023-10156-5. Online ahead of print.

Supplementation with the Probiotic Strains Bifidobacterium longum and Lactiplantibacillus rhamnosus Alleviates Glucose Intolerance by Restoring the IL-22 Response and Pancreatic Beta Cell Dysfunction in Type 2 Diabetic Mice

Won Jun Kim¹, Ri Ryu², Eun-Hee Doo^{2

3}, Yukyung Choi⁴, Kyunghwan Kim⁴, Byoung Kook Kim⁴, Heebal Kim^{1

5}, Myunghoo Kim⁶, Chul Sung Huh^{7

8}

Affiliations

¹ Department of Agricultural Biotechnology, College of Agriculture Sciences, Seoul National University, Seoul, South Korea.
² Institute of Green-Bio Science & Technology, Seoul National University, Pyeongchang, South Korea.
³ Department of Yuhan Biotechnology, School of Bio-Health Sciences, Yuhan University, Bucheon, 14780, South Korea.
⁴ Research Institute, Chong Kun Dang Bio Co. Ltd, Ansan, South Korea.
⁵ Department of Animal Science and Biotechnology, Seoul National University, Seoul, South Korea.
⁶ Department of Animal Science, Pusan National University, Miryang, South Korea. mhkim18@pusan.ac.kr.
⁷ Institute of Green-Bio Science & Technology, Seoul National University, Pyeongchang, South Korea. chulsunghuh@gmail.com.
⁸ Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, South Korea. chulsunghuh@gmail.com.

PMID: 37804432
DOI: 10.1007/s12602-023-10156-5

Supplementation with the Probiotic Strains Bifidobacterium longum and Lactiplantibacillus rhamnosus Alleviates Glucose Intolerance by Restoring the IL-22 Response and Pancreatic Beta Cell Dysfunction in Type 2 Diabetic Mice

Won Jun Kim et al. Probiotics Antimicrob Proteins. 2023.

. 2023 Oct 7.

doi: 10.1007/s12602-023-10156-5. Online ahead of print.

Authors

Won Jun Kim¹, Ri Ryu², Eun-Hee Doo^{2

3}, Yukyung Choi⁴, Kyunghwan Kim⁴, Byoung Kook Kim⁴, Heebal Kim^{1

5}, Myunghoo Kim⁶, Chul Sung Huh^{7

8}

Affiliations

¹ Department of Agricultural Biotechnology, College of Agriculture Sciences, Seoul National University, Seoul, South Korea.
² Institute of Green-Bio Science & Technology, Seoul National University, Pyeongchang, South Korea.
³ Department of Yuhan Biotechnology, School of Bio-Health Sciences, Yuhan University, Bucheon, 14780, South Korea.
⁴ Research Institute, Chong Kun Dang Bio Co. Ltd, Ansan, South Korea.
⁵ Department of Animal Science and Biotechnology, Seoul National University, Seoul, South Korea.
⁶ Department of Animal Science, Pusan National University, Miryang, South Korea. mhkim18@pusan.ac.kr.
⁷ Institute of Green-Bio Science & Technology, Seoul National University, Pyeongchang, South Korea. chulsunghuh@gmail.com.
⁸ Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, South Korea. chulsunghuh@gmail.com.

PMID: 37804432
DOI: 10.1007/s12602-023-10156-5

Abstract

Type 2 diabetes (T2D) is known as adult-onset diabetes, but recently, T2D has increased in the number of younger people, becoming a major clinical burden in human society. The objective of this study was to determine the effects of Bifidobacterium and Lactiplantibacillus strains derived from the feces of 20 healthy humans on T2D development and to understand the mechanism underlying any positive effects of probiotics. We found that Bifidobacterium longum NBM7-1 (Chong Kun Dang strain 1; CKD1) and Lactiplantibacillus rhamnosus NBM17-4 (Chong Kun Dang strain 2; CKD2) isolated from the feces of healthy Korean adults (n = 20) have anti-diabetic effects based on the insulin sensitivity. During the oral gavage for 8 weeks, T2D mice were supplemented with anti-diabetic drugs (1.0-10 mg/kg body weight) to four positive and negative control groups or four probiotics (200 uL; 1 × 10⁹ CFU/mL) to groups separately or combined to the four treatment groups (n = 6 per group). While acknowledging the relatively small sample size, this study provides valuable insights into the potential benefits of B. longum NBM7-1 and L. rhamnosus NBM17-4 in mitigating T2D development. The animal gene expression was assessed using a qRT-PCR, and metabolic parameters were assessed using an ELISA assay. We demonstrated that B. longum NBM7-1 in the CKD1 group and L. rhamnosus NBM17-4 in the CKD2 group alleviate T2D development through the upregulation of IL-22, which enhances insulin sensitivity and pancreatic functions while reducing liver steatosis. These findings suggest that B. longum NBM7-1 and L. rhamnosus NBM17-4 could be the candidate probiotics for the therapeutic treatments of T2D patients as well as the prevention of type 2 diabetes.

Keywords: Bifidobacterium longum; IL-22; Insulin sensitivity; Lactiplantibacillus rhamnosus; Type 2 diabetes (T2D).

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References

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[1] Zheng Y, Ley SH, Hu FB (2018) Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol 14(2):88–98. https://doi.org/10.1038/nrendo.2017.151 - DOI - PubMed

[2] Zheng Y, Ley SH, Hu FB (2018) Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol 14(2):88–98. https://doi.org/10.1038/nrendo.2017.151 - DOI - PubMed

[3] Wang X, Yang J, Qiu X, Wen Q, Liu M, Zhou D et al (2021) Probiotics, pre-biotics and synbiotics in the treatment of pre-diabetes: a systematic review of randomized controlled trials. Front Public Health 9:645035. https://doi.org/10.3389/fpubh.2021.645035 - DOI - PubMed - PMC

[4] Wang X, Yang J, Qiu X, Wen Q, Liu M, Zhou D et al (2021) Probiotics, pre-biotics and synbiotics in the treatment of pre-diabetes: a systematic review of randomized controlled trials. Front Public Health 9:645035. https://doi.org/10.3389/fpubh.2021.645035 - DOI - PubMed - PMC

[5] Mambiya M, Shang M, Wang Y, Li Q, Liu S, Yang L et al (2019) The play of genes and non-genetic factors on type 2 diabetes. Front Public Health 7:349. https://doi.org/10.3389/fpubh.2019.00349 - DOI - PubMed - PMC

[6] Mambiya M, Shang M, Wang Y, Li Q, Liu S, Yang L et al (2019) The play of genes and non-genetic factors on type 2 diabetes. Front Public Health 7:349. https://doi.org/10.3389/fpubh.2019.00349 - DOI - PubMed - PMC

[7] Foretz M, Guigas B, Bertrand L, Pollak M, Viollet B (2014) Metformin: from mechanisms of action to therapies. Cell Metab 20(6):953–966. https://doi.org/10.1016/j.cmet.2014.09.018 - DOI - PubMed

[8] Foretz M, Guigas B, Bertrand L, Pollak M, Viollet B (2014) Metformin: from mechanisms of action to therapies. Cell Metab 20(6):953–966. https://doi.org/10.1016/j.cmet.2014.09.018 - DOI - PubMed

[9] Bösenberg LH, van Zyl DG (2014) The mechanism of action of oral antidiabetic drugs: a review of recent literature. J Endocrinol Metab Diabetes S Afr 13(3):80–88. https://doi.org/10.1080/22201009.2008.10872177 - DOI

[10] Bösenberg LH, van Zyl DG (2014) The mechanism of action of oral antidiabetic drugs: a review of recent literature. J Endocrinol Metab Diabetes S Afr 13(3):80–88. https://doi.org/10.1080/22201009.2008.10872177 - DOI

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Supplementation with the Probiotic Strains Bifidobacterium longum and Lactiplantibacillus rhamnosus Alleviates Glucose Intolerance by Restoring the IL-22 Response and Pancreatic Beta Cell Dysfunction in Type 2 Diabetic Mice

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Supplementation with the Probiotic Strains Bifidobacterium longum and Lactiplantibacillus rhamnosus Alleviates Glucose Intolerance by Restoring the IL-22 Response and Pancreatic Beta Cell Dysfunction in Type 2 Diabetic Mice

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