A practitioner's view of spectral flow cytometry

doi:10.1038/s41592-023-02042-3

. 2024 May;21(5):740-743.

doi: 10.1038/s41592-023-02042-3.

A practitioner's view of spectral flow cytometry

Siddhartha Sharma¹, Josh Boyer¹, Luc Teyton²

Affiliations

¹ Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, USA.
² Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, USA. lteyton@scripps.edu.

PMID: 37789184
PMCID: PMC10991079 (available on 2025-05-01)
DOI: 10.1038/s41592-023-02042-3

A practitioner's view of spectral flow cytometry

Siddhartha Sharma et al. Nat Methods. 2024 May.

. 2024 May;21(5):740-743.

doi: 10.1038/s41592-023-02042-3.

Authors

Siddhartha Sharma¹, Josh Boyer¹, Luc Teyton²

Affiliations

¹ Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, USA.
² Department of Immunology and Microbiology, Scripps Research, La Jolla, CA, USA. lteyton@scripps.edu.

PMID: 37789184
PMCID: PMC10991079 (available on 2025-05-01)
DOI: 10.1038/s41592-023-02042-3

Abstract

Spectral flow cytometry allows the simultaneous analysis of a large number of cell surface markers at the single cell level

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Conflict of interest statement

Conflict of Interest Statement: The authors declare no conflict of interest.

Cited by

Development of a Spectral Flow Cytometry Analysis Pipeline for High-dimensional Immune Cell Characterization.
Vardaman D 3rd, Ali MA, Siam MHB, Bolding C, Tidwell H, Stephens HR, Patil M, Tyrrell DJ. Vardaman D 3rd, et al. J Immunol. 2024 Dec 1;213(11):1713-1724. doi: 10.4049/jimmunol.2400370. J Immunol. 2024. PMID: 39451039
Development of a Spectral Flow Cytometry Analysis Pipeline for High-Dimensional Immune Cell Characterization.
Vardaman D 3rd, Ali MA, Bolding C, Tidwell H, Stephens H, Tyrrell DJ. Vardaman D 3rd, et al. bioRxiv [Preprint]. 2024 Jun 22:2024.06.19.599633. doi: 10.1101/2024.06.19.599633. bioRxiv. 2024. Update in: J Immunol. 2024 Dec 1;213(11):1713-1724. doi: 10.4049/jimmunol.2400370 PMID: 38948780 Free PMC article. Updated. Preprint.

References

1. Nolan JP, Condello D. Spectral flow cytometry. Curr Protoc Cytom Chapter 1, Unit1 27 (2013). - PMC - PubMed
1. Han Y, Gu Y, Zhang AC, Lo YH. Review: imaging technologies for flow cytometry. Lab Chip 16, 4639–47 (2016). - PMC - PubMed
1. Turner JS, O’Halloran JA, Kalaidina E, Kim W, Schmitz AJ, Zhou JQ, Lei T, Thapa M, Chen RE, Case JB, Amanat F, Rauseo AM, Haile A, Xie X, Klebert MK, Suessen T, Middleton WD, Shi PY, Krammer F, Teefey SA, Diamond MS, Presti RM, Ellebedy AH. SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses. Nature (2021). - PMC - PubMed
1. Turner JS, Zhou JQ, Han J, Schmitz AJ, Rizk AA, Alsoussi WB, Lei T, Amor M, McIntire KM, Meade P, Strohmeier S, Brent RI, Richey ST, Haile A, Yang YR, Klebert MK, Suessen T, Teefey S, Presti RM, Krammer F, Kleinstein SH, Ward AB, Ellebedy AH. Human germinal centres engage memory and naive B cells after influenza vaccination. Nature 586, 127–32 (2020). - PMC - PubMed
1. Kreutmair S, Unger S, Nunez NG, Ingelfinger F, Alberti C, De Feo D, Krishnarajah S, Kauffmann M, Friebel E, Babaei S, Gaborit B, Lutz M, Jurado NP, Malek NP, Goepel S, Rosenberger P, Haberle HA, Ayoub I, Al-Hajj S, Nilsson J, Claassen M, Liblau R, Martin-Blondel G, Bitzer M, Roquilly A, Becher B. Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia. Immunity (2021). - PMC - PubMed

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[1] Nolan JP, Condello D. Spectral flow cytometry. Curr Protoc Cytom Chapter 1, Unit1 27 (2013). - PMC - PubMed

[2] Nolan JP, Condello D. Spectral flow cytometry. Curr Protoc Cytom Chapter 1, Unit1 27 (2013). - PMC - PubMed

[3] Han Y, Gu Y, Zhang AC, Lo YH. Review: imaging technologies for flow cytometry. Lab Chip 16, 4639–47 (2016). - PMC - PubMed

[4] Han Y, Gu Y, Zhang AC, Lo YH. Review: imaging technologies for flow cytometry. Lab Chip 16, 4639–47 (2016). - PMC - PubMed

[5] Turner JS, O’Halloran JA, Kalaidina E, Kim W, Schmitz AJ, Zhou JQ, Lei T, Thapa M, Chen RE, Case JB, Amanat F, Rauseo AM, Haile A, Xie X, Klebert MK, Suessen T, Middleton WD, Shi PY, Krammer F, Teefey SA, Diamond MS, Presti RM, Ellebedy AH. SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses. Nature (2021). - PMC - PubMed

[6] Turner JS, O’Halloran JA, Kalaidina E, Kim W, Schmitz AJ, Zhou JQ, Lei T, Thapa M, Chen RE, Case JB, Amanat F, Rauseo AM, Haile A, Xie X, Klebert MK, Suessen T, Middleton WD, Shi PY, Krammer F, Teefey SA, Diamond MS, Presti RM, Ellebedy AH. SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses. Nature (2021). - PMC - PubMed

[7] Turner JS, Zhou JQ, Han J, Schmitz AJ, Rizk AA, Alsoussi WB, Lei T, Amor M, McIntire KM, Meade P, Strohmeier S, Brent RI, Richey ST, Haile A, Yang YR, Klebert MK, Suessen T, Teefey S, Presti RM, Krammer F, Kleinstein SH, Ward AB, Ellebedy AH. Human germinal centres engage memory and naive B cells after influenza vaccination. Nature 586, 127–32 (2020). - PMC - PubMed

[8] Turner JS, Zhou JQ, Han J, Schmitz AJ, Rizk AA, Alsoussi WB, Lei T, Amor M, McIntire KM, Meade P, Strohmeier S, Brent RI, Richey ST, Haile A, Yang YR, Klebert MK, Suessen T, Teefey S, Presti RM, Krammer F, Kleinstein SH, Ward AB, Ellebedy AH. Human germinal centres engage memory and naive B cells after influenza vaccination. Nature 586, 127–32 (2020). - PMC - PubMed

[9] Kreutmair S, Unger S, Nunez NG, Ingelfinger F, Alberti C, De Feo D, Krishnarajah S, Kauffmann M, Friebel E, Babaei S, Gaborit B, Lutz M, Jurado NP, Malek NP, Goepel S, Rosenberger P, Haberle HA, Ayoub I, Al-Hajj S, Nilsson J, Claassen M, Liblau R, Martin-Blondel G, Bitzer M, Roquilly A, Becher B. Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia. Immunity (2021). - PMC - PubMed

[10] Kreutmair S, Unger S, Nunez NG, Ingelfinger F, Alberti C, De Feo D, Krishnarajah S, Kauffmann M, Friebel E, Babaei S, Gaborit B, Lutz M, Jurado NP, Malek NP, Goepel S, Rosenberger P, Haberle HA, Ayoub I, Al-Hajj S, Nilsson J, Claassen M, Liblau R, Martin-Blondel G, Bitzer M, Roquilly A, Becher B. Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia. Immunity (2021). - PMC - PubMed

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A practitioner's view of spectral flow cytometry

Affiliations

A practitioner's view of spectral flow cytometry

Authors

Affiliations

Abstract

Conflict of interest statement

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Cited by

References

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources