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. 2023:2702:3-12.
doi: 10.1007/978-1-0716-3381-6_1.

Antibody Phage Display

Alia Nur  1 , Maren Schubert  2 , Jing Yi Lai  1 , Michael Hust  2 , Yee Siew Choong  1 , Wan Yus Haniff Wan Isa  3 , Theam Soon Lim  4   5
Affiliations

Antibody Phage Display

Alia Nur et al. Methods Mol Biol. 2023.

Abstract

The application of antibodies has transcended across many areas of work but mainly as a research tool, for diagnostic and for therapeutic applications. Antibodies are immunoproteins from vertebrates that have the unique property of specifically binding foreign molecules and distinguish target antigens. This property allows antibodies to effectively protect the host from infections. Apart from the hybridoma technology using transgenic animals, antibody phage display is commonly considered the gold standard technique for the isolation of human monoclonal antibodies. The concept of antibody phage display surrounds the ability to display antibody fragments on the surface of M13 bacteriophage particles with the corresponding gene packaged within the particle. A repetitive in vitro affinity based selection process permits the enrichment of target specific binders. This process of recombinant human monoclonal antibody generation also enables additional engineering for various applications. This makes phage display an indispensable technique for antibody development and engineering activities.

Keywords: Antibody libraries; Biopanning; M13 bacteriophage; Monoclonal antibodies; Phage display.

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