At-Home Saliva Sampling in Healthy Adults Using CandyCollect, a Lollipop-Inspired Device
- PMID: 37364037
- PMCID: PMC10703142
- DOI: 10.1021/acs.analchem.3c00462
At-Home Saliva Sampling in Healthy Adults Using CandyCollect, a Lollipop-Inspired Device
Abstract
Respiratory infections are common in children, and there is a need for user-friendly collection methods. Here, we performed the first human subjects study using the CandyCollect device, a lollipop-inspired saliva collection device .We showed that the CandyCollect device can be used to collect salivary bacteria from healthy adults using Streptococcus mutans and Staphylococcus aureus as proof-of-concept commensal bacteria. We enrolled healthy adults in a nationwide (USA) remote study in which participants were sent study packages containing CandyCollect devices and traditional commercially available oral swabs and spit tubes. Participants sampled themselves at home, completed usability and user preference surveys, and mailed the samples back to our laboratory for analysis by qPCR. Our results showed that for participants in which a given bacterium (S. mutans or S. aureus) was detected in one or both of the commercially available methods (oral swab and/or spit tubes), CandyCollect devices had a 100% concordance with the positive result (n = 14 participants). Furthermore, the CandyCollect device was ranked the highest preference sampling method among the three sampling methods by 26 participants surveyed (combining survey results across two enrollment groups). We also showed that the CandyCollect device has a shelf life of up to 1 year at room temperature, a storage period that is convenient for clinics or patients to keep the CandyCollect device and use it any time. Taken together, we have demonstrated that the CandyCollect is a user-friendly saliva collection tool that has the potential to be incorporated into diagnostic assays in clinic visits and telemedicine.
Conflict of interest statement
CONFLICTS OF INTEREST
ABT has ownership in Stacks to the Future, LLC; ST: ownership in Stacks to the Future, LLC, Tasso, Inc., and Salus Discovery, LLC; and EB has ownership in Stacks to the Future, LLC, Tasso, Inc., and Salus Discovery, LLC and employment by Tasso, Inc. However, this research is not related to these companies.
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At-home saliva sampling in healthy adults using CandyCollect, a lollipop-inspired device.bioRxiv [Preprint]. 2023 May 10:2023.01.14.524039. doi: 10.1101/2023.01.14.524039. bioRxiv. 2023. Update in: Anal Chem. 2023 Jul 11;95(27):10211-10220. doi: 10.1021/acs.analchem.3c00462. PMID: 36711895 Free PMC article. Updated. Preprint.
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