Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Aug;54(4):313-327.
doi: 10.1007/s10735-023-10135-8. Epub 2023 Jun 21.

Knockdown of USF2 inhibits pyroptosis of podocytes and attenuates kidney injury in lupus nephritis

Ying Xie #  1   2 , Xiaoying Li #  3 , Wenli Deng #  3 , Nan Nan  4 , Huimei Zou  1   2   5 , Lei Gong  6 , Min Chen  1   2 , Jie Yu  1   2 , Peilei Chen  1   2 , Daolin Cui  7 , Fan Zhang  8   9
Affiliations

Knockdown of USF2 inhibits pyroptosis of podocytes and attenuates kidney injury in lupus nephritis

Ying Xie et al. J Mol Histol. 2023 Aug.

Abstract

As an essential factor in the prognosis of Systemic lupus erythematosus (SLE), lupus nephritis (LN) can accelerate the rate at which patients with SLE can transition to chronic kidney disease or even end-stage renal disease (ESRD). Proteinuria due to decreased glomerular filtration rate following podocyte injury is LN's most common clinical manifestation. Podocyte pyroptosis and related inflammatory factors in its process can promote lupus to involve kidney cells and worsen the occurrence and progression of LN, but its regulatory mechanism remains unknown. Accumulating evidence has shown that upstream stimulatory factor 2 (USF2) plays a vital role in the pathophysiology of kidney diseases. In this research, multiple experiments were performed to investigate the role of USF2 in the process of LN. USF2 was abnormally highly expressed in MRL/lpr mice kidney tissues. Renal function impairment and USF2 mRNA levels were positively correlated. Silencing of USF2 in MRL/lpr serum-stimulated cells significantly reduced serum-induced podocyte pyroptosis. USF2 enhanced NLRP3 expression at the transcriptional level. Silencing of USF2 in vivo attenuated kidney injury in MRL/lpr mice, which suggests that USF2 is important for LN development and occurrence.

Keywords: Lupus nephritis; Podocyte injury; Pyroptosis; Upstream stimulatory factor 2.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Amy H, Chan, Kate Schroder (2020) Inflammasome signaling and regulation of interleukin-1 family cytokines. J Exp Med 217(1):e20190314. https://doi.org/10.1084/jem.20190314 - DOI
    1. Anadi Mahajan J, Amelio K, Gairy G, Kaur, Roger A, Levy D, Roth, Damon B (2020) Systemic lupus erythematosus, lupus nephritis and end-stage renal disease: a pragmatic review mapping disease severity and progression. Lupus 29(9):1011–1020. https://doi.org/10.1177/0961203320932219 - DOI - PubMed - PMC
    1. Andrew S, Bomback, Glen S, Markowitz (2016) Lupus Podocytopathy: a distinct entity. Clin J Am Soc Nephrology: CJASN 11(4):547–548. https://doi.org/10.2215/CJN.01880216 - DOI
    1. Aringer M, Costenbader K, Daikh D, Brinks R, Mosca M, Ramsey-Goldman R, Smolen JS, David Wofsy (2019) 2019 European League Against Rheumatism/American College of Rheumatology classification criteria for systemic lupus erythematosus. Ann Rheum Dis 78(9):1151–1159. https://doi.org/10.1136/annrheumdis-2018-214819 - DOI - PubMed
    1. Austin HA 3rd, Lr M, Km J, Balow Je (1984) Diffuse proliferative lupus nephritis: identification of specific pathologic features affecting renal outcome. Kidney Int 25(4):689–695. https://doi.org/10.1038/ki.1984.75 - DOI - PubMed

Substances

LinkOut - more resources