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. 2023 Sep:109:110748.
doi: 10.1016/j.cellsig.2023.110748. Epub 2023 Jun 7.

MiR-200b-5p inhibits tumor progression in salivary adenoid cystic carcinoma via targeting BTBD1

Yuting Tang  1 , Qinghai Zhu  1 , Li Yang  2 , Ying Meng  2 , Gao Zhang  3 , Tian Zhou  2 , Chenxing Wang  1 , Xiaomeng Song  1 , Yu-Xiong Su  4 , Jinhai Ye  5
Affiliations

MiR-200b-5p inhibits tumor progression in salivary adenoid cystic carcinoma via targeting BTBD1

Yuting Tang et al. Cell Signal. 2023 Sep.

Abstract

Salivary adenoid cystic carcinoma (SACC) is a rare malignant tumor of the salivary gland. Studies have suggested that miRNA may play a crucial role in the invasion and metastasis of SACC. This study aimed to investigate the role of miR-200b-5p in SACC progression. Reverse transcription-quantitative PCR and western blot assay were used to detect the expression levels of miR-200b-5p and BTBD1. The biological functions of miR-200b-5p were evaluated via wound-healing assays, transwell assays, and xenograft nude mice model. The interaction between miR-200b-5p and BTBD1 was assessed using luciferase assay. Results showed that miR-200b-5p was downregulated in the SACC tissues while BTBD1 was upregulated. miR-200b-5p overexpression suppressed SACC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). Bioinformatics prediction and luciferase reporter assay revealed that miR-200b-5p could directly bind to BTBD1. Besides, miR-200b-5p overexpression could rescue the tumor-promoting effect of BTBD1. miR-200b-5p inhibited tumor progression by modulating EMT-related proteins, targeting BTBD1 and inhibiting PI3K/AKT signaling pathway. Overall, our findings indicate that miR-200b-5p can suppress SACC proliferation, migration, invasion, and EMT by regulating BTBD1 and PI3K/AKT axis, providing a promising therapeutic target for SACC treatment.

Keywords: BTBD1; PI3K/AKT; Salivary adenoid cystic carcinoma (SACC); Tumor progression; miR-200b-5p.

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Conflict of interest statement

Declaration of Competing Interest None.

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