Organization of the Hamster Cumulus Extracellular Matrix: A Hyaluronate-Glycoprotein Gel which Modulates Sperm Access to the Oocyte: Extracellular matrix/Hyaluronate/Oocyte-cumulus complex/Extracellular matrix glycoproteins/Sperm enzymes

doi:10.1111/j.1440-169X.1990.00353.x

. 1990 Aug;32(4):353-365.

doi: 10.1111/j.1440-169X.1990.00353.x.

Organization of the Hamster Cumulus Extracellular Matrix: A Hyaluronate-Glycoprotein Gel which Modulates Sperm Access to the Oocyte: Extracellular matrix/Hyaluronate/Oocyte-cumulus complex/Extracellular matrix glycoproteins/Sperm enzymes

Gary N Cherr¹, Ashley I Yudin², David F Katz^{2

3}

Affiliations

¹ Bodega Marine Laboratory, University of California at Davis, P.O. Box 247, Bodega Bay, California 94923.
² Division of Reproductive Biology, Department of Obstetrics and Gynecology, School of Medicine, Davis, California 95616.
³ Department of Chemical Engineering, University of California at Davis, Davis, California 95616.

PMID: 37280938
DOI: 10.1111/j.1440-169X.1990.00353.x

Organization of the Hamster Cumulus Extracellular Matrix: A Hyaluronate-Glycoprotein Gel which Modulates Sperm Access to the Oocyte: Extracellular matrix/Hyaluronate/Oocyte-cumulus complex/Extracellular matrix glycoproteins/Sperm enzymes

Gary N Cherr et al. Dev Growth Differ. 1990 Aug.

. 1990 Aug;32(4):353-365.

doi: 10.1111/j.1440-169X.1990.00353.x.

Authors

Gary N Cherr¹, Ashley I Yudin², David F Katz^{2

3}

Affiliations

¹ Bodega Marine Laboratory, University of California at Davis, P.O. Box 247, Bodega Bay, California 94923.
² Division of Reproductive Biology, Department of Obstetrics and Gynecology, School of Medicine, Davis, California 95616.
³ Department of Chemical Engineering, University of California at Davis, Davis, California 95616.

PMID: 37280938
DOI: 10.1111/j.1440-169X.1990.00353.x

Abstract

The mammalian oocyte-cumulus complex contains an extracellular matrix rich in hyaluronate. Recently, the microstructure of the hamster cumulus extracellular matrix was described (52). In the present work, we investigated the organization of this matrix. We employed freeze-substitution methodologies to investigate ultrastructural effects of various treatments, including sperm enzymes, on the matrix. Protease treatment resulted in disruption with a loss of the fibrillar structures and some expansion; in contrast, hyaluronidase treatment completely solubilized the matrix. EDTA extraction revealed that the fibrils are composed of fine filaments. A discrete region of the matrix immediately surrounding the oocyte, the corona radiata, was resistant to EDTA disruption. We found that hyaluronate is an ubiquitous constituent of the microstructural elements of this extracellular matrix. The matrix exhibits a carbohydrate:protein ratio of approximately 2:1. SDS-PAGE revealed that glycosylated polypeptides are bound to the matrix. The lectins LCA and WGA had differing affinities for these polypeptides, and bound ubiquitously to the intact matrix. The present data suggest that glycoprotein-hyaluronate interaction is critical for maintenance of the cumulus extracellular matrix microstructure and for its physical properties.

PubMed Disclaimer

Cited by

CD44 occupancy prevents macrophage multinucleation.
Sterling H, Saginario C, Vignery A. Sterling H, et al. J Cell Biol. 1998 Nov 2;143(3):837-47. doi: 10.1083/jcb.143.3.837. J Cell Biol. 1998. PMID: 9813101 Free PMC article.
Evolutionary, proteomic, and experimental investigations suggest the extracellular matrix of cumulus cells mediates fertilization outcomes†.
Keeble S, Firman RC, Sarver BAJ, Clark NL, Simmons LW, Dean MD. Keeble S, et al. Biol Reprod. 2021 Oct 11;105(4):1043-1055. doi: 10.1093/biolre/ioab082. Biol Reprod. 2021. PMID: 34007991 Free PMC article.
The extracellular matrix of porcine mature oocytes: origin, composition and presumptive roles.
Fléchon JE, Degrouard J, Kopecný V, Pivko J, Pavlok A, Motlik J. Fléchon JE, et al. Reprod Biol Endocrinol. 2003 Dec 14;1:124. doi: 10.1186/1477-7827-1-124. Reprod Biol Endocrinol. 2003. PMID: 14675483 Free PMC article.

References

1. Arnott, S., A. K. Mitra. 1984. X-Ray diffraction analysis of glycosaminoglycans. In: Molecular biophysics of the extracellular matrix, S. Arnott, D. A. Rees, E. R. Morris, (eds), Humana Press, Clifton , New Jersey , pp. 41-67.
1. Arsenault, L. A., F. P. Ottensmeyer, and I. B. Heath. 1988. An electron microscopic and spectroscopic study of murine epiphyseal cartilage: analysis of fine structure and matrix vesicles preserved by slam freezing and freeze substitution. J. Ultrast. Molec. Struct. Res. 98, 32-47.
1. Ball, G. D., M. E. Bellin, R. L. Ax, and N. L. First. 1982. Glycosaminoglycans in bovine cumulus-oocyte complexes: Morphology and chemistry. Mol Cell Endocrinol. 28, 113-122.
1. Bavister, B. 1986. Animal in vitro fertilization and embryo development. In: Manipulation of Mammalian Development, R. B. L. Gwatkin, (ed), Plenum Press, New York , pp 81-148.
1. Bradley, M. P., and D. L. Garbers. 1983. The stimulation of bovine caudal epididymal sperm forward motility by bovine cumulus egg complex in vitro. Biochem. Biophys Res. Commun. 115, 777-787.

LinkOut - more resources

Full Text Sources
- Wiley
Research Materials
- NCI CPTC Antibody Characterization Program

[1] Arnott, S., A. K. Mitra. 1984. X-Ray diffraction analysis of glycosaminoglycans. In: Molecular biophysics of the extracellular matrix, S. Arnott, D. A. Rees, E. R. Morris, (eds), Humana Press, Clifton , New Jersey , pp. 41-67.

[2] Arnott, S., A. K. Mitra. 1984. X-Ray diffraction analysis of glycosaminoglycans. In: Molecular biophysics of the extracellular matrix, S. Arnott, D. A. Rees, E. R. Morris, (eds), Humana Press, Clifton , New Jersey , pp. 41-67.

[3] Arsenault, L. A., F. P. Ottensmeyer, and I. B. Heath. 1988. An electron microscopic and spectroscopic study of murine epiphyseal cartilage: analysis of fine structure and matrix vesicles preserved by slam freezing and freeze substitution. J. Ultrast. Molec. Struct. Res. 98, 32-47.

[4] Arsenault, L. A., F. P. Ottensmeyer, and I. B. Heath. 1988. An electron microscopic and spectroscopic study of murine epiphyseal cartilage: analysis of fine structure and matrix vesicles preserved by slam freezing and freeze substitution. J. Ultrast. Molec. Struct. Res. 98, 32-47.

[5] Ball, G. D., M. E. Bellin, R. L. Ax, and N. L. First. 1982. Glycosaminoglycans in bovine cumulus-oocyte complexes: Morphology and chemistry. Mol Cell Endocrinol. 28, 113-122.

[6] Ball, G. D., M. E. Bellin, R. L. Ax, and N. L. First. 1982. Glycosaminoglycans in bovine cumulus-oocyte complexes: Morphology and chemistry. Mol Cell Endocrinol. 28, 113-122.

[7] Bavister, B. 1986. Animal in vitro fertilization and embryo development. In: Manipulation of Mammalian Development, R. B. L. Gwatkin, (ed), Plenum Press, New York , pp 81-148.

[8] Bavister, B. 1986. Animal in vitro fertilization and embryo development. In: Manipulation of Mammalian Development, R. B. L. Gwatkin, (ed), Plenum Press, New York , pp 81-148.

[9] Bradley, M. P., and D. L. Garbers. 1983. The stimulation of bovine caudal epididymal sperm forward motility by bovine cumulus egg complex in vitro. Biochem. Biophys Res. Commun. 115, 777-787.

[10] Bradley, M. P., and D. L. Garbers. 1983. The stimulation of bovine caudal epididymal sperm forward motility by bovine cumulus egg complex in vitro. Biochem. Biophys Res. Commun. 115, 777-787.

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Organization of the Hamster Cumulus Extracellular Matrix: A Hyaluronate-Glycoprotein Gel which Modulates Sperm Access to the Oocyte: Extracellular matrix/Hyaluronate/Oocyte-cumulus complex/Extracellular matrix glycoproteins/Sperm enzymes

Affiliations

Organization of the Hamster Cumulus Extracellular Matrix: A Hyaluronate-Glycoprotein Gel which Modulates Sperm Access to the Oocyte: Extracellular matrix/Hyaluronate/Oocyte-cumulus complex/Extracellular matrix glycoproteins/Sperm enzymes

Authors

Affiliations

Abstract

Similar articles

Cited by

References

LinkOut - more resources

Full Text Sources

Research Materials