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. 2023:2676:265-284.
doi: 10.1007/978-1-0716-3251-2_19.

Noncanonical Amino Acid Incorporation in Mice

Zhetao Zheng  1 , Qing Xia  2
Affiliations

Noncanonical Amino Acid Incorporation in Mice

Zhetao Zheng et al. Methods Mol Biol. 2023.

Abstract

Genetic code expansion enables in cellulo biosynthesis of curative proteins with enhanced specificity, improved stability, and even novel functions, due to the incorporation of artificial, designed, noncanonical amino acids (ncAAs). In addition, this orthogonal system also holds great potential for in vivo suppressing nonsense mutations during protein translation, providing an alternative strategy for alleviating inherited diseases caused by premature termination codons (PTCs). Here we describe the approach to explore the therapeutic efficacy and long-term safety of this strategy in transgenic mdx mice with stably expanded genetic codes. Theoretically, this method is applicable to about 11% of monogenic diseases involving nonsense mutations.

Keywords: Gene targeting; Genetic code expansion; Non-canonical amino acids; Nonsense mutation diseases; Translation machinery.

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References

    1. Young DD, Schultz PG (2018) Playing with the molecules of life. ACS Chem Biol 13:854–870 - DOI - PubMed - PMC
    1. de la Torre D, Chin JW (2021) Reprogramming the genetic code. Nat Rev Genet 22:169–184 - DOI - PubMed
    1. Wan W, Tharp JM, Liu WR (2014) Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool. Biochim Biophys Acta (BBA) Proteins Proteomics 1844:1059–1070 - DOI - PubMed
    1. Shi N, Tong L, Lin H et al (2022) Optimizing eRF1 to enable the genetic encoding of three distinct noncanonical amino acids in mammalian cells. Adv Biol 6(11):2200092 - DOI
    1. Ernst RJ, Krogager TP, Maywood ES et al (2016) Genetic code expansion in the mouse brain. Nat Chem Biol 12:776–778 - DOI - PubMed - PMC

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