Denitrification shifted autotroph-heterotroph interactions in Microcystis aggregates
- PMID: 37257745
- DOI: 10.1016/j.envres.2023.116269
Denitrification shifted autotroph-heterotroph interactions in Microcystis aggregates
Abstract
Denitrification is the most important process for nitrogen removal in eutrophic lakes and was mostly investigated in lake sediment. Denitrification could also be mediated by cyanobacterial aggregates, yet how this process impacts nitrogen (N) availability and the associated autotroph-heterotroph relationships within cyanobacterial aggregates has not been investigated. In this study, incubation experiments with nitrate amendment were conducted with Microcystis aggregates (MAs). Measurement of nitrogen contents, 16S rRNA-based microbial community profiling and metatranscriptomic sequencing were used to jointly assess nitrogen turnover dynamics, as well as changes in microbial composition and gene expression. Strong denitrification potential was revealed, and maximal N removal was achieved within two days, after which the communities entered a state of severe N limitation. Changes of active microbial communities were further promoted both with regard to taxonomic composition and transcriptive activities. Expression of transportation-related genes confirmed competition for N sources by Microcystis and phycospheric communities. Strong stress response to reactive oxygen species by Microcystis was revealed. Notably, interspecific relationships among Microcystis and phycospheric communities exhibited a shift toward antagonistic interactions, particularly evidenced by overall increased expression of genes related to cell lysis and utilization of cellular materials. Patterns of fatty acid and starch metabolism also suggested changes in carbon metabolism and cross-feeding patterns within MAs. Taken together, this study demonstrated substantial denitrification potential of MAs, which, importantly, further induced changes in both metabolic activities and autotroph-heterotroph interactions. These findings also highlight the key role of nutrient condition in shaping autotroph-heterotroph relationships.
Keywords: Autotroph-heterotroph interactions; Cyanobacterial aggregates; Denitrification; Eutrophication; Microcystis.
Copyright © 2023 Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Similar articles
-
Metatranscriptomics analysis of cyanobacterial aggregates during cyanobacterial bloom period in Lake Taihu, China.Environ Sci Pollut Res Int. 2018 Feb;25(5):4811-4825. doi: 10.1007/s11356-017-0733-4. Epub 2017 Dec 3. Environ Sci Pollut Res Int. 2018. PMID: 29198031
-
Determinants of Total and Active Microbial Communities Associated with Cyanobacterial Aggregates in a Eutrophic Lake.mSystems. 2023 Apr 27;8(2):e0099222. doi: 10.1128/msystems.00992-22. Epub 2023 Mar 16. mSystems. 2023. PMID: 36927063 Free PMC article.
-
The secretion of organics by living Microcystis under the dark/anoxic condition and its enhancing effect on nitrate removal.Chemosphere. 2018 Apr;196:280-287. doi: 10.1016/j.chemosphere.2017.12.197. Epub 2018 Jan 2. Chemosphere. 2018. PMID: 29306780
-
The facilitating role of phycospheric heterotrophic bacteria in cyanobacterial phosphonate availability and Microcystis bloom maintenance.Microbiome. 2023 Jun 26;11(1):142. doi: 10.1186/s40168-023-01582-2. Microbiome. 2023. PMID: 37365664 Free PMC article.
-
Microcystis spp. and phosphorus in aquatic environments: A comprehensive review on their physiological and ecological interactions.Sci Total Environ. 2023 Jun 20;878:163136. doi: 10.1016/j.scitotenv.2023.163136. Epub 2023 Mar 30. Sci Total Environ. 2023. PMID: 37001662 Review.
Cited by
-
Biological and Chemical Approaches for Controlling Harmful Microcystis Blooms.J Microbiol. 2024 Mar;62(3):249-260. doi: 10.1007/s12275-024-00115-2. Epub 2024 Apr 8. J Microbiol. 2024. PMID: 38587591 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources