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Review
. 2023 Apr 6;11(4):1107.
doi: 10.3390/biomedicines11041107.

Transcriptomic Approaches in Studies on and Applications of Chimeric Antigen Receptor T Cells

Karolina Pierzynowska  1 , Lidia Gaffke  1 , Jan M Zaucha  2 , Grzegorz Węgrzyn  1
Affiliations
Review

Transcriptomic Approaches in Studies on and Applications of Chimeric Antigen Receptor T Cells

Karolina Pierzynowska et al. Biomedicines. .

Abstract

Chimeric antigen receptor T (CAR-T) cells are specifically modified T cells which bear recombinant receptors, present at the cell surface and devoted to detect selected antigens of cancer cells, and due to the presence of transmembrane and activation domains, able to eliminate the latter ones. The use of CAR-T cells in anti-cancer therapies is a relatively novel approach, providing a powerful tool in the fight against cancer and bringing new hope for patients. However, despite huge possibilities and promising results of preclinical studies and clinical efficacy, there are various drawbacks to this therapy, including toxicity, possible relapses, restrictions to specific kinds of cancers, and others. Studies desiring to overcome these problems include various modern and advanced methods. One of them is transcriptomics, a set of techniques that analyze the abundance of all RNA transcripts present in the cell at certain moment and under certain conditions. The use of this method gives a global picture of the efficiency of expression of all genes, thus revealing the physiological state and regulatory processes occurring in the investigated cells. In this review, we summarize and discuss the use of transcriptomics in studies on and applications of CAR-T cells, especially in approaches focused on improved efficacy, reduced toxicity, new target cancers (like solid tumors), monitoring the treatment efficacy, developing novel analytical methods, and others.

Keywords: CAR-T cells; anti-cancer therapies; cancer; transcriptomics.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic representation of the various possibilities of applications of transcriptomic methods in studies on and practical use of CAR-T cells, reported to date in the literature, as depicted in the form of the Cyske rosette chart (a kind of the schematic summary as proposed by Cyske et al. [85]).
See this image and copyright information in PMC

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This research received no external funding.

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