doi: 10.1093/nar/14.11.4617.
Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance
- PMID: 3714487
- PMCID: PMC311469
- DOI: 10.1093/nar/14.11.4617
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Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance
Nucleic Acids Res.
.
Free PMC article
Abstract
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.
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