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. 2023 Apr 8;14(4):881.
doi: 10.3390/genes14040881.

Identification of Key Functional Genes and LncRNAs Influencing Muscle Growth and Development in Leizhou Black Goats

Xiuhui Zhao  1   2   3 , Junning Ye  1   2   3 , Xunkai Lin  1 , Huiwen Xue  1 , Xian Zou  2 , Guangbin Liu  1   3 , Ming Deng  1   3 , Baoli Sun  1   3 , Yongqing Guo  1   3 , Dewu Liu  1   3 , Yaokun Li  1   3
Affiliations

Identification of Key Functional Genes and LncRNAs Influencing Muscle Growth and Development in Leizhou Black Goats

Xiuhui Zhao et al. Genes (Basel). .

Abstract

Meat yield and quality are important economic traits of livestock. Herein, longissimus dorsi (LD) muscles of Leizhou black goats aged 0, 3, and 6 months were used to identify differentially expressed messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) by high-throughput RNA sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to analyze differentially expressed genes. Expression levels of regulator of calcineurin 1 (RCAN1) and olfactory receptor 2AP1 (OR2AP1) were significantly different in LD muscles of goats aged 0, 3, and 6 months, indicating potentially important roles in postnatal muscle development. Differentially expressed lncRNAs and mRNAs were mainly enriched in biological processes and pathways related to cellular energy metabolism, consistent with previous studies. Three lncRNAs, TCONS_00074191, TCONS_00074190, and TCONS_00078361, may play a cis-acting role with methyltransferase-like 11B (METTL11B) genes and participate in the methylation of goat muscle proteins. Some of the identified genes may provide valuable resources for future studies on postnatal meat development in goat muscles.

Keywords: Leizhou black goat; lncRNA; longissimus dorsi; mRNA; meat quality; meat yield; protein methylation; skeletal muscle.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Goat skeletal muscle structure and conservation. ORF stands for open reading frame.
Figure 2
Figure 2
qRT-PCR results for mRNAs and lncRNAs and FPKM from RNA-seq of M0, M3, and M6 groups.
See this image and copyright information in PMC

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Grants and funding

This work was supported by a grant from the Modern Agricultural Industrial Technology System of Guangdong Province (grant number 2021KJ127).