Transcriptome analysis reveals the regulatory effects of Bacillus amyloliquefaciens and Bacillus pumilus on immune and digestive related genes in the spleen of weanling black goats

doi:10.1007/s10142-023-01025-z

. 2023 Apr 14;23(2):124.

doi: 10.1007/s10142-023-01025-z.

Transcriptome analysis reveals the regulatory effects of Bacillus amyloliquefaciens and Bacillus pumilus on immune and digestive related genes in the spleen of weanling black goats

Nanchi Zhang^{1

2}, Juan Li^{1

2}, Li Wang^{3

4}, Yong Wei⁵, Quzhe Emu⁶, Feng Xu⁶, Lin Zhang⁶

Affiliations

¹ Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Chengdu, 610041, China.
² Key Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, No.16, South Fourth Section, First Ring Road, Chengdu, 610041, China.
³ Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Chengdu, 610041, China. qinxin916@aliyun.com.
⁴ Key Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, No.16, South Fourth Section, First Ring Road, Chengdu, 610041, China. qinxin916@aliyun.com.
⁵ Animal Genetics and Breeding Key Laboratory of Sichuan Province, Sichuan Animal Sciences Academy, No.7, Niusha Road, Chengdu, 610066, China. veishangyan@163.com.
⁶ Animal Genetics and Breeding Key Laboratory of Sichuan Province, Sichuan Animal Sciences Academy, No.7, Niusha Road, Chengdu, 610066, China.

PMID: 37055595
DOI: 10.1007/s10142-023-01025-z

Transcriptome analysis reveals the regulatory effects of Bacillus amyloliquefaciens and Bacillus pumilus on immune and digestive related genes in the spleen of weanling black goats

Nanchi Zhang et al. Funct Integr Genomics. 2023.

. 2023 Apr 14;23(2):124.

doi: 10.1007/s10142-023-01025-z.

Authors

Nanchi Zhang^{1

2}, Juan Li^{1

2}, Li Wang^{3

4}, Yong Wei⁵, Quzhe Emu⁶, Feng Xu⁶, Lin Zhang⁶

Affiliations

¹ Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Chengdu, 610041, China.
² Key Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, No.16, South Fourth Section, First Ring Road, Chengdu, 610041, China.
³ Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Chengdu, 610041, China. qinxin916@aliyun.com.
⁴ Key Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, No.16, South Fourth Section, First Ring Road, Chengdu, 610041, China. qinxin916@aliyun.com.
⁵ Animal Genetics and Breeding Key Laboratory of Sichuan Province, Sichuan Animal Sciences Academy, No.7, Niusha Road, Chengdu, 610066, China. veishangyan@163.com.
⁶ Animal Genetics and Breeding Key Laboratory of Sichuan Province, Sichuan Animal Sciences Academy, No.7, Niusha Road, Chengdu, 610066, China.

PMID: 37055595
DOI: 10.1007/s10142-023-01025-z

Abstract

The aim of the present study was to evaluate the effects of Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 on the expressions of spleen genes in weanling Jintang black goats. Bacillus amyloliquefaciens fsznc-06 (BA-treated group) and Bacillus pumilus fsznc-09 (BP-treated group) were directly fed to goats, and the spleens were harvested for transcriptome analysis. The KEGG pathway analysis showed that the differentially expressed genes (DEGs) in BA-treated vs CON group were mainly involved in digestive system and immune system, while those in BP-treated vs CON group were mainly involved in immune system, and those in BA-treated vs BP-treated group were mainly involved in digestive system. In conclusion, Bacillus amyloliquefaciens fsznc-06 might promote the expressions of genes related to immune system and digestive system, reduce the expressions of disease genes related to digestive system and might promote mutual accommodation of some immune genes in weanling black goat. Bacillus pumilus fsznc-09 might promote the expressions of genes related to immune system and mutual accommodation of some immune genes in weanling black goat. Bacillus amyloliquefaciens fsznc-06 has advantages over Bacillus pumilus fsznc-09 in promoting the expressions of genes related to digestive system and mutual accommodation of some immune genes.

Keywords: Bacillus amyloliquefaciens; Bacillus pumilus; Jintang black goat; Spleen; Transcriptome analysis.

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References

1. Bindiya ES, Tina KJ, Sasidharan RS, Bhat SG (2019) BaCf3: highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens. 3 Biotech 9(4):136. https://doi.org/10.1007/s13205-019-1639-2 - DOI - PubMed - PMC
1. Chien CC, Lin TY, Chi CC, Liu CH (2020) Probiotic, Bacillus subtilis E20 alters the immunity of white shrimp, Litopenaeus vannamei via glutamine metabolism and hexosamine biosynthetic pathway. Fish Shellfish Immunol 98:176–185. https://doi.org/10.1016/j.fsi.2020.01.014 - DOI - PubMed
1. Chu J, Wang Y, Zhao B, Zhang XM, Liu K, Mao L, Kalamiyets E (2019) Isolation and identification of new antibacterial compounds from Bacillus pumilus. Appl Microbiol Biotechnol 103(20):8375–8381. https://doi.org/10.1007/s00253-019-10083-y - DOI - PubMed
1. Dindhoria K, Monga I, Thind AS (2022) Computational approaches and challenges for identification and annotation of non-coding RNAs using RNA-Seq. Funct Integr Genomics 22(6):1105–1112. https://doi.org/10.1007/s10142-022-00915-y - DOI - PubMed
1. Fang H, Shi H, Li X, Sun D, Li F, Li B, Ding Y, Ma Y, Liu Y, Zhang Y, Shen L, Bai Y, Yang Y, Lu J (2015) Exercise intolerance and developmental delay associated with a novel mitochondrial ND5 mutation. Sci Rep 5:10480. https://doi.org/10.1038/srep10480 - DOI - PubMed - PMC

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[1] Bindiya ES, Tina KJ, Sasidharan RS, Bhat SG (2019) BaCf3: highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens. 3 Biotech 9(4):136. https://doi.org/10.1007/s13205-019-1639-2 - DOI - PubMed - PMC

[2] Bindiya ES, Tina KJ, Sasidharan RS, Bhat SG (2019) BaCf3: highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens. 3 Biotech 9(4):136. https://doi.org/10.1007/s13205-019-1639-2 - DOI - PubMed - PMC

[3] Chien CC, Lin TY, Chi CC, Liu CH (2020) Probiotic, Bacillus subtilis E20 alters the immunity of white shrimp, Litopenaeus vannamei via glutamine metabolism and hexosamine biosynthetic pathway. Fish Shellfish Immunol 98:176–185. https://doi.org/10.1016/j.fsi.2020.01.014 - DOI - PubMed

[4] Chien CC, Lin TY, Chi CC, Liu CH (2020) Probiotic, Bacillus subtilis E20 alters the immunity of white shrimp, Litopenaeus vannamei via glutamine metabolism and hexosamine biosynthetic pathway. Fish Shellfish Immunol 98:176–185. https://doi.org/10.1016/j.fsi.2020.01.014 - DOI - PubMed

[5] Chu J, Wang Y, Zhao B, Zhang XM, Liu K, Mao L, Kalamiyets E (2019) Isolation and identification of new antibacterial compounds from Bacillus pumilus. Appl Microbiol Biotechnol 103(20):8375–8381. https://doi.org/10.1007/s00253-019-10083-y - DOI - PubMed

[6] Chu J, Wang Y, Zhao B, Zhang XM, Liu K, Mao L, Kalamiyets E (2019) Isolation and identification of new antibacterial compounds from Bacillus pumilus. Appl Microbiol Biotechnol 103(20):8375–8381. https://doi.org/10.1007/s00253-019-10083-y - DOI - PubMed

[7] Dindhoria K, Monga I, Thind AS (2022) Computational approaches and challenges for identification and annotation of non-coding RNAs using RNA-Seq. Funct Integr Genomics 22(6):1105–1112. https://doi.org/10.1007/s10142-022-00915-y - DOI - PubMed

[8] Dindhoria K, Monga I, Thind AS (2022) Computational approaches and challenges for identification and annotation of non-coding RNAs using RNA-Seq. Funct Integr Genomics 22(6):1105–1112. https://doi.org/10.1007/s10142-022-00915-y - DOI - PubMed

[9] Fang H, Shi H, Li X, Sun D, Li F, Li B, Ding Y, Ma Y, Liu Y, Zhang Y, Shen L, Bai Y, Yang Y, Lu J (2015) Exercise intolerance and developmental delay associated with a novel mitochondrial ND5 mutation. Sci Rep 5:10480. https://doi.org/10.1038/srep10480 - DOI - PubMed - PMC

[10] Fang H, Shi H, Li X, Sun D, Li F, Li B, Ding Y, Ma Y, Liu Y, Zhang Y, Shen L, Bai Y, Yang Y, Lu J (2015) Exercise intolerance and developmental delay associated with a novel mitochondrial ND5 mutation. Sci Rep 5:10480. https://doi.org/10.1038/srep10480 - DOI - PubMed - PMC

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Transcriptome analysis reveals the regulatory effects of Bacillus amyloliquefaciens and Bacillus pumilus on immune and digestive related genes in the spleen of weanling black goats

Affiliations

Transcriptome analysis reveals the regulatory effects of Bacillus amyloliquefaciens and Bacillus pumilus on immune and digestive related genes in the spleen of weanling black goats

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Abstract

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