Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

doi:10.3389/fmicb.2023.1097905

Review

. 2023 Mar 9:14:1097905.

doi: 10.3389/fmicb.2023.1097905. eCollection 2023.

Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Jinghua Pan¹, Mengyi Zeng¹, Mengmeng Zhao^{1

2}, Liangzong Huang^{1

2}

Affiliations

¹ School of Life Science and Engineering, Foshan University, Foshan, China.
² Veterinary Teaching Hospital, Foshan University, Foshan, China.

PMID: 36970703
PMCID: PMC10033578
DOI: 10.3389/fmicb.2023.1097905

Review

Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Jinghua Pan et al. Front Microbiol. 2023.

. 2023 Mar 9:14:1097905.

doi: 10.3389/fmicb.2023.1097905. eCollection 2023.

Authors

Jinghua Pan¹, Mengyi Zeng¹, Mengmeng Zhao^{1

2}, Liangzong Huang^{1

2}

Affiliations

¹ School of Life Science and Engineering, Foshan University, Foshan, China.
² Veterinary Teaching Hospital, Foshan University, Foshan, China.

PMID: 36970703
PMCID: PMC10033578
DOI: 10.3389/fmicb.2023.1097905

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes clinical syndromes typified as reproductive disorders in sows and respiratory diseases in piglets. PRRSV remains one of the most prevalent pathogens affecting the pig industry, because of its complex infection profile and highly heterogeneous genetic and recombination characteristics. Therefore, a rapid and effective PRRSV detection method is important for the prevention and control of PRRS. With extensive in-depth research on PRRSV detection methods, many detection methods have been improved and promoted. Laboratory methods include techniques based on virus isolation (VI), enzyme-linked immunosorbent assays (ELISA), indirect immunofluorescence assays (IFA), immunoperoxidase monolayer assays (IPMA), polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), digital PCR (dPCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), clustered regularly interspaced short palindromic repeats (CRISPR), metagenomic next-generation sequencing (mNGS), and other methods. This study reviews the latest research on improving the main PRRSV detection methods and discusses their advantages and disadvantages.

Keywords: metagenomic next-generation sequencing; molecular biology; porcine reproductive and respiratory syndrome; serological assay; virus isolation.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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References

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[1] Abd El Wahed A., El-Deeb A., El-Tholoth M., Abd El Kader H., Ahmed A., Hassan S., et al. . (2013). A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. PLoS One 8:e71642. doi: 10.1371/journal.pone.0071642, PMID: - DOI - PMC - PubMed

[2] Abd El Wahed A., El-Deeb A., El-Tholoth M., Abd El Kader H., Ahmed A., Hassan S., et al. . (2013). A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. PLoS One 8:e71642. doi: 10.1371/journal.pone.0071642, PMID: - DOI - PMC - PubMed

[3] Arvidsson S., Kwasniewski M., Riaño-Pachón D. M., Mueller-Roeber B. (2008). QuantPrime--a flexible tool for reliable high-throughput primer Design for Quantitative PCR. BMC Bioinform. 9:465. doi: 10.1186/1471-2105-9-465 - DOI - PMC - PubMed

[4] Arvidsson S., Kwasniewski M., Riaño-Pachón D. M., Mueller-Roeber B. (2008). QuantPrime--a flexible tool for reliable high-throughput primer Design for Quantitative PCR. BMC Bioinform. 9:465. doi: 10.1186/1471-2105-9-465 - DOI - PMC - PubMed

[5] Bi C., Shao Z., Zhang Y., Hu L., Li J., Huang L., et al. . (2017). Identification of a linear B-cell epitope on non-structural protein 12 of porcine reproductive and respiratory syndrome virus using a monoclonal antibody. Arch. Virol. 162, 2239–2246. doi: 10.1007/s00705-017-3355-8, PMID: - DOI - PubMed

[6] Bi C., Shao Z., Zhang Y., Hu L., Li J., Huang L., et al. . (2017). Identification of a linear B-cell epitope on non-structural protein 12 of porcine reproductive and respiratory syndrome virus using a monoclonal antibody. Arch. Virol. 162, 2239–2246. doi: 10.1007/s00705-017-3355-8, PMID: - DOI - PubMed

[7] Boyle D. S., Lehman D. A., Lillis L., Peterson D., Singhal M., Armes N., et al. . (2013). Rapid detection of HIV-1 Proviral DNA for early infant diagnosis using recombinase polymerase amplification. mBio 4, e00113–e00135. doi: 10.1128/mBio.00135-13 - DOI - PMC - PubMed

[8] Boyle D. S., Lehman D. A., Lillis L., Peterson D., Singhal M., Armes N., et al. . (2013). Rapid detection of HIV-1 Proviral DNA for early infant diagnosis using recombinase polymerase amplification. mBio 4, e00113–e00135. doi: 10.1128/mBio.00135-13 - DOI - PMC - PubMed

[9] Brockmeier S. L., Loving C. L., Vorwald A. C., Kehrli M. E., Baker R. B., Nicholson T. L., et al. . (2012). Genomic sequence and virulence comparison of four type 2 porcine reproductive and respiratory syndrome virus strains. Virus Res. 169, 212–221. doi: 10.1016/j.virusres.2012.07.030, PMID: - DOI - PubMed

[10] Brockmeier S. L., Loving C. L., Vorwald A. C., Kehrli M. E., Baker R. B., Nicholson T. L., et al. . (2012). Genomic sequence and virulence comparison of four type 2 porcine reproductive and respiratory syndrome virus strains. Virus Res. 169, 212–221. doi: 10.1016/j.virusres.2012.07.030, PMID: - DOI - PubMed

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Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Affiliations

Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus

Authors

Affiliations

Abstract

Conflict of interest statement

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Abstract

Conflict of interest statement

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