Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Sep;18(9):1940-1941.
doi: 10.4103/1673-5374.363827.

Tissue optical clearing for neural regeneration research

Tingting Yu  1 , Jianyi Xu  1 , Dan Zhu  1
Affiliations

Tissue optical clearing for neural regeneration research

Tingting Yu et al. Neural Regen Res. 2023 Sep.
No abstract available

PubMed Disclaimer

Conflict of interest statement

None

Figures

Figure 1
Figure 1
Overview of tissue optical clearing scheme for 3D imaging and its applications in neural tissues and target organs. (A) Overview of tissue optical clearing scheme for three-dimensional (3D) imaging. The main steps include whole-mount fluorescent labeling, tissue clearing, 3D imaging with optical sectioning microscopy, and data analysis of image stacks. (B) Tissue optical clearing provides novel tools for 3D morphological analyses of neural tissues and target organs for outcome assessments in neural regeneration research, such as the 3D visualization of the spinal cord (central nervous system [CNS]), dorsal root ganglions and nerves (peripheral nervous system [PNS]), as well as the skin and skeletal muscle (target organs). Images are adapted with permissions from Jing et al. (2018), Qi et al. (2019), and Daeschler et al. (2022).
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Daeschler SC, Zhang J, Gordon T, Borschel GH. Optical tissue clearing enables rapid, precise and comprehensive assessment of three-dimensional morphology in experimental nerve regeneration research. Neural Regen Res. 2022;17:1348–1356. - PMC - PubMed
    1. Ertürk A, Mauch CP, Hellal F, Förstner F, Keck T, Becker K, Jährling N, Steffens H, Richter M, Hübener M, Kramer E, Kirchhoff F, Dodt HU, Bradke F. Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury. Nat Med. 2012;18:166–171. - PubMed
    1. Fogli B, Corthout N, Kerstens A, Bosse F, Klimaschewski L, Munck S, Schweigreiter R. Imaging axon regeneration within synthetic nerve conduits. Sci Rep. 2019;9:10095. - PMC - PubMed
    1. Hilton BJ, Blanquie O, Tedeschi A, Bradke F. High-resolution 3D imaging and analysis of axon regeneration in unsectioned spinal cord with or without tissue clearing. Nat Protoc. 2019;14:1235–1260. - PubMed
    1. Jing D, Zhang S, Luo W, Gao X, Men Y, Ma C, Liu X, Yi Y, Bugde A, Zhou BO, Zhao Z, Yuan Q, Feng JQ, Gao L, Ge WP, Zhao H. Tissue clearing of both hard and soft tissue organs with the PEGASOS method. Cell Res. 2018;28:803–818. - PMC - PubMed