Expression of a single transfected cDNA converts fibroblasts to myoblasts
- PMID: 3690668
- DOI: 10.1016/0092-8674(87)90585-x
Expression of a single transfected cDNA converts fibroblasts to myoblasts
Abstract
5-azacytidine treatment of mouse C3H10T1/2 embryonic fibroblasts converts them to myoblasts at a frequency suggesting alteration of one or only a few closely linked regulatory loci. Assuming such loci to be differentially expressed as poly(A)+ RNA in proliferating myoblasts, we prepared proliferating myoblast-specific, subtracted cDNA probes to screen a myocyte cDNA library. Based on a number of criteria, three cDNAs were selected and characterized. We show that expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts. Myogenesis also occurs, but to a lesser extent, when this cDNA is expressed in a number of other cell lines. The major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family.
Comment in
-
From MYOD1 to iPS cells.Nat Rev Mol Cell Biol. 2010 Dec;11(12):817. doi: 10.1038/nrm3018. Nat Rev Mol Cell Biol. 2010. PMID: 21102604 No abstract available.
Similar articles
-
A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.Genes Dev. 1989 May;3(5):628-40. doi: 10.1101/gad.3.5.628. Genes Dev. 1989. PMID: 2473006
-
Transfection of a DNA locus that mediates the conversion of 10T1/2 fibroblasts to myoblasts.Cell. 1986 Dec 5;47(5):649-56. doi: 10.1016/0092-8674(86)90507-6. Cell. 1986. PMID: 2430720
-
MyoD1: a nuclear phosphoprotein requiring a Myc homology region to convert fibroblasts to myoblasts.Science. 1988 Oct 21;242(4877):405-11. doi: 10.1126/science.3175662. Science. 1988. PMID: 3175662
-
An avian muscle factor related to MyoD1 activates muscle-specific promoters in nonmuscle cells of different germ-layer origin and in BrdU-treated myoblasts.Genes Dev. 1989 Jul;3(7):986-96. doi: 10.1101/gad.3.7.986. Genes Dev. 1989. PMID: 2777078
-
Characterisation of a genomic clone covering the structural mouse MyoD1 gene and its promoter region.Nucleic Acids Res. 1991 Dec 11;19(23):6433-9. doi: 10.1093/nar/19.23.6433. Nucleic Acids Res. 1991. PMID: 1754380 Free PMC article.
Cited by
-
Computational methods for direct cell conversion.Cell Cycle. 2016 Dec 16;15(24):3343-3354. doi: 10.1080/15384101.2016.1238119. Epub 2016 Oct 13. Cell Cycle. 2016. PMID: 27736295 Free PMC article. Review.
-
Spatial re-organization of myogenic regulatory sequences temporally controls gene expression.Nucleic Acids Res. 2015 Feb 27;43(4):2008-21. doi: 10.1093/nar/gkv046. Epub 2015 Feb 4. Nucleic Acids Res. 2015. PMID: 25653159 Free PMC article.
-
Chemical transdifferentiation: closer to regenerative medicine.Front Med. 2016 Jun;10(2):152-65. doi: 10.1007/s11684-016-0445-z. Epub 2016 May 3. Front Med. 2016. PMID: 27142989 Review.
-
Switching cell fate, ncRNAs coming to play.Cell Death Dis. 2013 Jan 17;4(1):e464. doi: 10.1038/cddis.2012.196. Cell Death Dis. 2013. PMID: 23328671 Free PMC article. Review.
-
Skeletal muscle hypertrophy and regeneration: interplay between the myogenic regulatory factors (MRFs) and insulin-like growth factors (IGFs) pathways.Cell Mol Life Sci. 2013 Nov;70(21):4117-30. doi: 10.1007/s00018-013-1330-4. Epub 2013 Apr 4. Cell Mol Life Sci. 2013. PMID: 23552962 Free PMC article. Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases