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. 2023 Feb 24;13(5):873.
doi: 10.3390/diagnostics13050873.

Diagnosis of Dengue Virus Infections Imported to Hungary and Phylogenetic Analysis of Virus Isolates

Orsolya Nagy  1   2 , Anna Nagy  1 , Anita Koroknai  1 , Nikolett Csonka  1 , Mária Takács  1   2
Affiliations

Diagnosis of Dengue Virus Infections Imported to Hungary and Phylogenetic Analysis of Virus Isolates

Orsolya Nagy et al. Diagnostics (Basel). .

Abstract

Background: Dengue virus is one of the most important arbovirus infections of public health concern. Between 2017 and June 2022, 75 imported dengue infections were confirmed by laboratory diagnostic methods in Hungary. Our study aimed to isolate the imported Dengue strains and characterize them by whole-genome sequencing.

Methods: Laboratory diagnosis of imported infections was carried out using both serological and molecular methods. Virus isolation was attempted on Vero E6 cell lines. An in-house amplicon-based whole-genome sequencing method was applied for the detailed molecular characterization of the isolated virus strains.

Results: From 75 confirmed Dengue infected patients, 68 samples were used for virus isolation. Isolation and whole-genome sequencing were successful in the case of eleven specimens. Isolated strains belonged to Dengue-1,-2,-3 serotypes.

Discussion: The isolated strains corresponded to the circulating genotypes of the visited geographic area, and some of the genotypes were linked with more severe DENV cases in the literature. We found that multiple factors, including viral load, specimen type, and patient antibody status, influence the isolation efficacy.

Conclusions: Analysis of imported DENV strains can help estimate the outcomes of a possible local DENV transmission in Hungary, a threat from the near future.

Keywords: Dengue virus; imported arbovirus infection; virus isolation; whole-genome sequencing.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Tested patients and confirmed DENV cases/year between 2017 and June 2022.
Figure 2
Figure 2
Countries visited by confirmed DENV-infected patients 3 weeks before symptom onset.
Figure 3
Figure 3
Maximum likelihood phylogenetic tree of DENV-1 strains. Isolates of imported DENV infections to Hungary marked by blue rhombuses.
Figure 4
Figure 4
Maximum likelihood phylogenetic tree of DENV-2 strains. Isolates of imported DENV infections to Hungary marked by green circles.
Figure 5
Figure 5
Maximum likelihood phylogenetic tree of DENV-3 strains. Isolates of imported DENV infections to Hungary marked by orange squares.
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