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. 2023 Apr;37(4):910-913.
doi: 10.1038/s41375-023-01842-y. Epub 2023 Mar 8.

The fatty acid elongase Elovl6 is crucial for hematopoietic stem cell engraftment and leukemia propagation

Yusuke Kiyoki #  1 , Takayasu Kato #  2   3 , Sakura Kito  1 , Takashi Matsuzaka  4   5 , Shin Morioka  6   7 , Junko Sasaki  6   7   8 , Kenichi Makishima  1 , Tatsuhiro Sakamoto  9 , Hidekazu Nishikii  9 , Naoshi Obara  9 , Mamiko Sakata-Yanagimoto  9   10 , Takehiko Sasaki  6   7 , Hitoshi Shimano  5 , Shigeru Chiba  11
Affiliations

The fatty acid elongase Elovl6 is crucial for hematopoietic stem cell engraftment and leukemia propagation

Yusuke Kiyoki et al. Leukemia. 2023 Apr.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. Elovl6 is essential for hematopoietic stem cell (HSC) repopulation and acute myeloid leukemia (AML) propagation by MLL::AF9-immortalized (MA9) cells in mice.
A Results of competitive repopulation assay. Shown are PB chimerism of Ly5.2 cells after transplantation with 1 × 106 Ly5.2 WT (n = 9) or E6KO (n = 15) test cells and 1 × 106 Ly5.1/5.2 competitor BM cells (i) or transplantation with 2 × 106 Ly5.2 WT (n = 9) or E6KO (n = 15) test cells and 2 × 105 Ly5.1/5.2 competitor BM cells (ii). B Survival of mice transplanted with WT or E6KO MA9 cells. Representative data of three independent experiments are shown. C Transwell chemotaxis assay. Percent migration of WT or E6KO CD34-LSK cells (control; n = 5 each. CXCL12; n = 9 each.) (i) and WT or E6KO MA9 cells (n = 3 each) (ii) toward CXCL12-containing or control chambers. Effect of ELOVL6 inhibitor on migration of WT or E6KO MA9 cells toward a CXCL12-containing chamber; n = 3 each (iii). Data are shown as the mean ± SD in all bar charts. *P < 0.05, **P < 0.01, ***P < 0.001.
Fig. 2
Fig. 2. ELOVL6 regulates chemotaxis via PI3K-RAC signaling following CXCL12 stimulation and is involved in mouse AML.
A Phosphorylation of AKT in wild-type (WT) or E6KO MA9 cells before and after CXCL12 stimulation. Representative western blot (i) and results of three experiments (ii). Pull-down assay for GTP-bound Rac1 in WT or E6KO MA9 cells before and after CXCL12 stimulation. Representative western blot (iii) and results of four experiments (iv). B Effect of Copanlisib (i), MK2206 (ii), and EHT1864 (iii) on migration of WT or E6KO MA9 cells toward a CXCL12-containing chamber; n = 3–5 each. C Survival of mice transplanted with E6KO MA9Flag-E6 and E6KO MA9mock cells.
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