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. 2023 Feb 12;12(1):19.
doi: 10.3390/biotech12010019.

Fabrication of a Polycaprolactone/Chitosan Nanofibrous Scaffold Loaded with Nigella sativa Extract for Biomedical Applications

Qasim Shakir Kahdim  1   2 , Najmeddine Abdelmoula  2 , Hassan Al-Karagoly  3 , Salim Albukhaty  4   5 , Jabbar Al-Saaidi  3
Affiliations

Fabrication of a Polycaprolactone/Chitosan Nanofibrous Scaffold Loaded with Nigella sativa Extract for Biomedical Applications

Qasim Shakir Kahdim et al. BioTech (Basel). .

Abstract

In this study, biocompatible electrospun nanofiber scaffolds were produced using poly(-caprolactone (PCL)/chitosan (CS) and Nigella sativa (NS) seed extract, and their potential for biomedical applications was investigated. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), total porosity measurements, and water contact angle measurements were used to evaluate the electrospun nanofibrous mats. Additionally, the antibacterial activities of Escherichia coli and Staphylococcus aureus were investigated, as well as cell cytotoxicity and antioxidant activity, using MTT and DPPH assays, respectively. The obtained PCL/CS/NS nanofiber mat was observed by SEM to have a homogeneous and bead-free morphology, with average diameters of 81.19 ± 4.38 nm. Contact angle measurements showed that the wettability of the electrospun PCL/Cs fiber mats decreased with the incorporation of NS when compared to the PCL/CS nanofiber mats. Efficient antibacterial activity against S. aureus and E. coli was displayed, and an in vitro cytotoxic assay demonstrated that the normal murine fibroblast cell line (L929 cells) remained viable after 24, 48, and 72 h following direct contact with the produced electrospun fiber mats. The results suggest that the PCL/CS/NS hydrophilic structure and the densely interconnected porous design are biocompatible materials, with the potential to treat and prevent microbial wound infections.

Keywords: Nigella Sativa; biocompatibility; chitosan; electrospinning; polycaprolactone.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
GC–MS chromatogram of the ethanol extract of Nigella sativa seeds.
Figure 2
Figure 2
Electrospun PCL/CS/NS (mean diameter 81.19 ± 4.38 nm) in a SEM photograph: (A) PCL (1 µm); (B) PCL/CS/NS (1 µm); (C) PCL/CS/NS (500 nm); (D) The diameter size distributions.
Figure 3
Figure 3
FTIR spectrum of PCL/CS/NS, PCL, CS, and NS extract.
Figure 4
Figure 4
(A) L929 cell viability percentages during different periods of time (24, 48, and 72 h). (B) L929 cytotoxicity percentages during different periods of time (24, 48, and 72 h).
Figure 5
Figure 5
Antimicrobial activity of PCL/CS/NS nanofibers against S. aureus and E. coli. Inhibition zones (mm): 1—antibiotic; 2—CS/NS; 3—PCL/CS; 4—PCL/CS/NS nanofibers; 5—PCL; 6—negative control (D.W.).
Figure 6
Figure 6
Percentage inhibition of DPPH radical in the presence of different concentrations of CS, NS, PCL/CS/NS nanofiber mats, and ascorbic acid.
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References

    1. Medeiros G.B., Lima F.d.A., de Almeida D.S., Guerra V.G., Aguiar M.L. Modification and Functionalization of Fibers Formed by Electrospinning: A Review. Membranes. 2022;12:861. doi: 10.3390/membranes12090861. - DOI - PMC - PubMed
    1. Xue J., Wu T., Dai Y., Xia Y. Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications. Chem. Rev. 2019;119:5298–5415. - PMC - PubMed
    1. Albukhaty S., Al-Karagoly H., Allafchian A.R., Jalali S.A.H., Al-Kelabi T., Muhannad M. Production and characterization of biocompatible nanofibrous scaffolds made of β-sitosterol loaded polyvinyl alcohol/tragacanth gum composites. Nanotechnology. 2021;33:085102. doi: 10.1088/1361-6528/ac3789. - DOI - PubMed
    1. Castro K.C., Campos M.G.N., Mei L.H.I. Hyaluronic Acid Electrospinning: Challenges, Applications in Wound Dressings and New Perspectives. Int. J. Biol. Macromol. 2021;173:251–266. doi: 10.1016/j.ijbiomac.2021.01.100. - DOI - PubMed
    1. Han D.K., Park K.D., Hubbell J.A., Kim Y.H. Surface Characteristics and Biocompatibility of Lactide-Based Poly(Ethylene Glycol) Scaffolds for Tissue Engineering. J. Biomater. Sci. Polym. Ed. 1998;9:667–680. - PubMed

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