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. 2023 Jan-Mar;106(1):368504221148843.
doi: 10.1177/00368504221148843.

Evolutionary analysis and expression profiling of the HSP70 gene family in response to abiotic stresses in tomato (Solanum lycopersicum)

Nam Tuan Vu  1   2 , Ngoc Bich Thi Nguyen  2 , Hanh Hong Ha  2 , Linh Nhat Nguyen  2 , Ly Han Luu  2 , Ha Quang Dao  2 , Trinh Thi Vu  2 , Hue Thu Thi Huynh  1   2 , Hien Thu Thi Le  1   2
Affiliations

Evolutionary analysis and expression profiling of the HSP70 gene family in response to abiotic stresses in tomato (Solanum lycopersicum)

Nam Tuan Vu et al. Sci Prog. 2023 Jan-Mar.

Abstract

Heat shock protein 70 (HSP70) genes play essential roles in guarding plants against abiotic stresses, including heat, drought, and salt. In this study, the SlHSP70 gene family in tomatoes has been characterized using bioinformatic tools. 25 putative SlHSP70 genes in the tomato genome were found and classified into five subfamilies, with multi-subcellular localizations. Twelve pairs of gene duplications were identified, and segmental events were determined as the main factor for the gene family expansion. Based on public RNA-seq data, gene expression analysis identified the majority of genes expressed in the examined organelles. Further RNA-seq analysis and then quantitative RT-PCR validation showed that many SlHSP70 members are responsible for cellular feedback to heat, drought, and salt treatments, in which, at least five genes might be potential key players in the stress response. Our results provided a thorough overview of the SlHSP70 gene family in the tomato, which may be useful for the evolutionary and functional analysis of SlHSP70 under abiotic stress conditions.

Keywords: Abiotic stress; HSP70; evolutionary; gene expression; heat shock protein; tomato.

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Conflict of interest statement

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Phylogenetic analysis of SlHSP70 based on their amino acid sequences. (a) The analysis within the SlHSP70 gene family. (b) The comparative analysis of HSP70s between tomato and potato, Arabidopsis and rice.
Figure 2.
Figure 2.
The distribution and duplication of the SlHSP70 gene family. The blue lines indicate the SlHSP70 gene duplication pairs.
Figure 3.
Figure 3.
PlantCARE analysis of different cis-elements presented in the promoter regions of the SlHSP70 genes.
Figure 4.
Figure 4.
The express pattern of SlHSP70s in various tissues of tomato in normal condition. Heat map represented the log2 of FPKM value for RNA-seq data of SlHSP70 expressions from the tomato eFP browser database.
Figure 5.
Figure 5.
RNA-seq analysis of SlHSP70 gene family under heat, drought and salt treatments. Heat map represented the log2 of normalized FPKM value for RNA-seq data of SlHSP70s expression under heat, drought (from GEO database, accession no. GSE151277) and salt treatments (accession no. GSE148353).
Figure 6.
Figure 6.
Real-time PCR validation of SlHSP70s expression under heat (a), drought (b) and salt (c) stresses. The Bar graph with the error bar represented the Mean ± SEM of the relative expression level of targeted genes from three biological replications using UBQ3 as a reference gene. The mean expression level of SlHSP70 genes under stresses was compared to control using Student's t-test with * for P < 0.05, and ** for P < 0.01.
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