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. 2023 Jan 6;37(10):e371003.
doi: 10.1590/acb371003. eCollection 2023.

The antifibrotic and anti-inflammatory effects of FZHY prescription on the kidney in rats after unilateral ureteral obstruction

Ziwei Chen  1 , Shaobo Wu  2 , Yu Zeng  3 , Xueying Li  2 , Mengping Wang  2 , Zejun Chen  4 , Ming Chen  2
Affiliations

The antifibrotic and anti-inflammatory effects of FZHY prescription on the kidney in rats after unilateral ureteral obstruction

Ziwei Chen et al. Acta Cir Bras. .

Abstract

Purpose: To explore the potential impact of traditional Chinese herb FuZhengHuaYuJiangZhuTongLuo recipe (FZHY) on renal interstitial fibrosis (RIF) in chronic kidney disease (CKD) at cellular and molecular levels.

Methods: Unilateral ureteral obstruction (UUO) rats were established as the RIF model in vivo. The rats were given intragastric administration with FZHY once a day for consecutive 7, 14 and 21 days, respectively. The renal function parameters and inflammation indicators in kidney tissues were measured using enzyme-linked immunosorbent assay, the CD4+/CD8+ T cells in peripheral blood was detected using flow cytometry, the renal fibrosis degree was estimated using Masson's staining, and the fibrosis-related genes' expression was detected using quantitative polymerase chain reaction, western blotting, and immunohistochemistry analyses.

Results: FZHY prescription reduced the serum creatinine and blood urea nitrogen, decreased the levels of c-reactive protein, interleukin-1, interleukin-6 and tumor necrosis factor-α in kidney tissues, and increased the ratio of CD4+/CD8+ T cells in peripheral blood. FZHY prescription suppressed the renal tissue fibrosis and reduced the levels of laminin, fibronectin, collagen I and collagen III.

Conclusions: FZHY prescription suppressed the renal fibrosis and improved the condition of "Healthy Qi Deficiency and Evil Qi Excess" in rats with UUO, which may provide an effective method for CKD treatment.

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Conflict of interest statement

Conflict of interest: Nothing to declare.

Figures

Figure 1
Figure 1. FZHY alleviated the renal injury in UUO rats. The amount of serum creatinine (SCR) and blood urea nitrogen (BUN) for rats in sham, UUO, UUO + FZHY and UUO + AST-120 groups on (a, b; n = 5) day 7, (c, d; n = 5) day 14, and (e, n = 3; f, n = 5) day 21. (g) The renal fibrosis assessment was detected by the Masson’s trichrome staining (400 × magnification) on day 7, day 14 and day 21, separately.
Figure 2
Figure 2. FZHY inhibited the inflammatory response in UUO rats. The inflammation markers such as CRP, IL-1, IL-6 and TNF-α were assessed in sham group, UUO model group, UUO + FZHY and UUO + AST-120 groups on (a; CRP, n = 3; IL-1, n = 3; IL-6, n = 4; TNF-α, n = 4) day 7, (b; CRP, n = 4; IL-1, n = 3; IL-6, n = 3; TNF-α, n = 4) day 14, and (c; CRP, n = 4; IL-1, n = 4; IL-6, n = 4; TNF-α, n = 3) day 21.
Figure 3
Figure 3. FZHY changed the function of the immune system in UUO rats. The ratio of CD4+/CD8+ T cells was determined in sham group, UUO model group and UUO + FZHY group on (a, n = 3) day 7, (b, n = 3) day 14, and (c, n = 2) day 21, and the histogram and statistical test for the ratio of CD4+/CD8+ were performed by GraphPad software on day 7, day 14, and day 21, respectively.
Figure 4
Figure 4. FZHY reduced the mRNA and protein levels of the renal interstitial fibrosis markers in UUO rats. The (a, n = 3) mRNA and (b, n = 3) protein levels of the renal interstitial fibrosis markers LN, FN, Col-I and Col-III were evaluated in sham, UUO, UUO + FZHY and UUO + AST-120 groups on day 14 and day 21.
Figure 5
Figure 5. The renal interstitial fibrosis markers in UUO rats were detected by immunohistochemical staining. The immunohistochemical staining of LN, FN, Col-I and Col-III (×400) were detected in sham group (n = 3), UUO model group (n = 3), FZHY-treated group (n = 3) and AST-120-treated group (n = 3) on day 14 and day 21.
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