Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec 23;13(1):57.
doi: 10.3390/ani13010057.

Longitudinal Survey on aMPV Circulation in French Broiler Flocks following Different Vaccination Strategies

Caterina Lupini  1 , Claudia Maria Tucciarone  2 , Giulia Mescolini  1 , Giulia Quaglia  1 , Giulia Graziosi  1 , Vincent Turblin  3 , Paul Brown  4 , Mattia Cecchinato  2 , Matteo Legnardi  2 , Thomas Delquigny  5 , Stéphane Lemiere  5 , Guillaume Perreul  5 , Elena Catelli  1
Affiliations

Longitudinal Survey on aMPV Circulation in French Broiler Flocks following Different Vaccination Strategies

Caterina Lupini et al. Animals (Basel). .

Abstract

In recent years, the impact of respiratory disease resulting from Avian Metapneumovirus (aMPV) infection has been generally rising in the broiler industry in Europe. In this context, in order to investigate aMPV contribution to the clinical picture and the potential benefits of diversified vaccination strategies compared to nonvaccination policies, a longitudinal monitoring was performed, also evaluating Infectious Bronchitis Virus (IBV) presence. Broiler flocks located in Western France, where aMPV has already proven to be a health and productivity issue, were screened by RT-PCR on rhino-pharyngeal swabs, and the viruses were genetically characterized by sequence analysis. For a more comprehensive picture of aMPV molecular epidemiology and evolution in France, aMPV subtype B strains detected from 1985 to 1998 were sequenced and included in the analysis. The survey confirmed the detection of aMPV subtype B in commercial broiler flocks in France, together with a certain heterogeneity demonstrated by the circulation of more recent and historical French field strains. No IBV field strains were detected. The implementation and evaluation of different management choices and vaccine strategies suggests once again that immunization does not prevent infection but contributes greatly to the containment of the clinical manifestations.

Keywords: France; avian metapneumovirus; broiler; infectious bronchitis virus; longitudinal study; phylogenetic analysis.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Results of aMPV diagnostic monitoring.
Figure 2
Figure 2
Phylogenetic tree based on G gene nucleotide sequences of French aMPV-B strains. The analysis included B strains detected in broiler longitudinal studies (shown in bold), historical French B field strains, contemporary selected European B strains [13] and B vaccines most commonly used (marked with a green triangle). The evolutionary history was inferred using the maximum likelihood method and Kimura 2-parameter model implemented in MEGA X. All nucleotide positions containing gaps and missing data were eliminated. There were a total of 288 positions in the final dataset. Only bootstrap values ≥ 70% are reported.
Figure 3
Figure 3
Results of IBV monitoring.
Figure 4
Figure 4
Phylogenetic tree reconstructed using IBV strain references from Valastro et al. (2016) [34]. French strains identified in the present study are marked with a black circle. The tree was reconstructed using the maximum likelihood method and general time reversible model with gamma distribution. Bootstrap support (>70%) is shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 123 nucleotide sequences and a total of 295 positions in the final dataset.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Suarez D.L., Miller P.J., Koch G., Mundt E., Rautenschlein S. Diseases of Poultry. Wiley; Hoboken, NJ, USA: 2020. Newcastle Disease, Other Avian Paramyxoviruses, and Avian Metapneumovirus Infections; pp. 109–166.
    1. Picault J.P., Giraud P., Drouin P., Guittet M., Bennejean G., Lamande J., Toquin D., Gueguen C. Isolation of a TRTV-like virus from chickens with swollen-head syndrome. Vet. Rec. 1987;121:135. doi: 10.1136/vr.121.6.135-a. - DOI - PubMed
    1. Toquin D., Bäyon-Auboyer M.H., Eterradossi N., Jestin V., Morin H. Isolation of a pneumovirus from a Muscovy duck. Vet. Rec. 1999;145:680. - PubMed
    1. Catelli E., De Marco M.A., Delogu M., Terregino C., Guberti V. Serological evidence of avian pneumovirus infection in reared and free-living pheasants. Vet. Rec. 2001;149:56–58. doi: 10.1136/vr.149.2.56. - DOI - PubMed
    1. Cecchinato M., Lupini C., Silveira F., Listorti V., Mescolini G., Morandini E., Franzo G., Catelli E. Molecular characterization of avian metapneumovirus from Guinea fowls (Numida meleagridis) Pak. Vet. J. 2018;38:419–423. doi: 10.29261/pakvetj/2018.088. - DOI

Grants and funding

This research was partially funded from Boehringer Ingelheim Santé Animale, 69007, Lyon, France.

LinkOut - more resources