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Review
. 2022 Dec 20:17:6491-6511.
doi: 10.2147/IJN.S382115. eCollection 2022.

Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration

Qi You #  1   2 , Minxun Lu #  1   2 , Zhuangzhuang Li  1   2 , Yong Zhou  1   2 , Chongqi Tu  1   2
Affiliations
Review

Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration

Qi You et al. Int J Nanomedicine. .

Abstract

Bone defects that are congenital or the result of infection, malignancy, or trauma represent a challenge to the global healthcare system. To address this issue, multiple research groups have been developing novel cell sheet technology (CST)-based approaches to promote bone regeneration. These methods hold promise for use in regenerative medicine because they preserve cell-cell contacts, cell-extracellular matrix interactions, and the protein makeup of cell membranes. This review introduces the concept and preparation system of the cell sheet (CS), explores the application of CST in bone regeneration, highlights the current states of the bone regeneration via CST, and offers perspectives on the challenges and future research direction of translating current knowledge from the lab to the clinic.

Keywords: bone defect; bone regeneration; bone tissue engineering; cell sheet technology.

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Conflict of interest statement

There is no conflict of interest.

Figures

Figure 1
Figure 1
Schematic illustration of cell sheet formation and harvesting.
Figure 2
Figure 2
Schematic illustration of light-induced cell sheet (CS) preparation and harvest. (A) Ultraviolet 254 (UV254)-induced cell sheet patterning on titanium oxide (TiO2) nanodots film (TNF). (B) UV365-induced anisotropic cell sheet (ACS) detachment on TNF. (C) The procedure for reactive oxygen species (ROS) -induced CS transfer from Hp-PK film to fibrin gel, and then the stacking process under green light. (D) A schematic illustration for the harvest of multiple CSs by near-infrared (NIR) light. (E) A schematic illustration for the precisely directed CS detachment from the gradient photothermal surface.
Figure 3
Figure 3
Schematic illustration of the fabricated 3D vascularized heterotypic cell sheet by magnetic responsive system. MSCs, mesenchymal stem cells. MNPs, magnetite nanoparticles. HUVECs, human umbilical vein endothelial cells.
Figure 4
Figure 4
Cell sheet (CS)-detaching process by ultrasonic vibration. (A) Schematic illustration of CS detachment. (B) The CS was detached from the bottom of the dish by ultrasonic vibration. (C) CS-detaching system in an incubator.
Figure 5
Figure 5
The types of constructions and in vivo models used in bone regeneration.
See this image and copyright information in PMC

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Grants and funding

The work was supported by the Science and Technology Research Program of Sichuan Province (2020YFS0036), QingDao research institutes of SiChuan University, Research of biomedical materials and 3D printing related products (20GZ30301), China postdoctoral Science Foundation (2021M702342) and 1·3·5 project for disciplines of excellence, West China Hospital, Sichuan University (ZYJC18017).