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Review
. 2022 Nov 23;14(23):5746.
doi: 10.3390/cancers14235746.

Ten Years of CRISPRing Cancers In Vitro

Davide Capoferri  1 , Serena Filiberti  1 , Jessica Faletti  1 , Camilla Tavani  1 , Roberto Ronca  1
Affiliations
Review

Ten Years of CRISPRing Cancers In Vitro

Davide Capoferri et al. Cancers (Basel). .

Abstract

Cell lines have always constituted a good investigation tool for cancer research, allowing scientists to understand the basic mechanisms underlying the complex network of phenomena peculiar to the transforming path from a healthy to cancerous cell. The introduction of CRISPR in everyday laboratory activity and its relative affordability greatly expanded the bench lab weaponry in the daily attempt to better understand tumor biology with the final aim to mitigate cancer's impact in our lives. In this review, we aim to report how this genome editing technique affected in the in vitro modeling of different aspects of tumor biology, its several declinations, and analyze the advantages and drawbacks of each of them.

Keywords: CRISPR; cancer; genome editing; hallmark; in vitro; technology.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Classic representation of cancer hallmarks according to Hanahan’s latest review (2022). Surrounding the graphic, tables listing the genome editing targets described in the corresponding section and the effect they had in vitro according to the corresponding hallmark.
See this image and copyright information in PMC

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