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. 2022 Nov 15;20(11):714.
doi: 10.3390/md20110714.

Structural Characterization and In Vivo Anti-Inflammatory Activity of Fucoidan from Cystoseira crinita (Desf.) Borry

Elisaveta Apostolova  1 , Paolina Lukova  2 , Alexandra Baldzhieva  3   4 , Cédric Delattre  5   6 , Roland Molinié  7 , Emmanuel Petit  7 , Redouan Elboutachfaiti  7 , Mariana Nikolova  8 , Ilia Iliev  8 , Marianna Murdjeva  3   4 , Vesela Kokova  1
Affiliations

Structural Characterization and In Vivo Anti-Inflammatory Activity of Fucoidan from Cystoseira crinita (Desf.) Borry

Elisaveta Apostolova et al. Mar Drugs. .

Abstract

The aim of this study was to evaluate the effects of fucoidan isolated from C. crinita on histamine-induced paw inflammation in rats, and on the serum levels of TNF-α, IL-1β, IL-6, and IL-10 in rats during systemic inflammation response. The levels of TNF-α in a model of acute peritonitis in rats were also investigated. The isolated crude fucoidan was identified as a sulfated xylogalactofucan with high, medium, and low molecular weight fractions and a content of fucose of 39.74%, xylose of 20.75%, and galactose of 15.51%. Fucoidan from C. crinita showed better anti-inflammatory effects in the rat paw edema model, and this effect was present during all stages of the experiment. When compared to controls, a commercial fucoidan from F. vesiculosus, the results also displayed anti-inflammatory activity on the 60th, 90th, and 120th minute of the experiment. A significant decrease in serum levels of IL-1β in rats treated with both doses of C. crinita fucoidan was observed in comparison to controls, whereas TNF-α concentrations were reduced only in the group treated with fucoidan from C. crinita at the dose of 25 mg/kg bw. In the model of carrageenan-induced peritonitis, we observed a tendency of decrease in the levels of the pro-inflammatory cytokine TNF-α in peritoneal fluid after a single dose of C. crinita fucoidan, but this did not reach the statistical significance margin. Single doses of C. crinita fucoidan did not alter serum levels of the anti-inflammatory cytokine IL-10 in animals with lipopolysaccharide-induced systemic inflammation.

Keywords: Cystoseira crinita; IL-1β; TNF-α; anti-inflammatory effect; cytokines; fucoidan; peritonitis; rat paw edema.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
FTIR of fucoidan extracted from C. crinita.
Figure 2
Figure 2
1H NMR spectra of the sulfated fucoidan polysaccharides (a) C. crinita crude fucoidan and (b) commercial standard fucoidan from F. vesiculosus at 80 °C in D2O solution.
Figure 3
Figure 3
SEC-MALS chromatogram of C. crinita crude fucoidan giving Mw (g/mol) versus V (mL) (black), RI signal (blue), and light scattering at 90° (red).
Figure 4
Figure 4
Effects of diclofenac, fucoidan standard from F.vesiculosus (50 mg/kg bw), and fucoidan test from C. crinita (25 and 50 mg/kg bw) on paw edema induced by histamine in rats. * p < 0.05 vs. controls at the same time; ** p < 0.01 vs. controls at the same time; *** p < 0.001 vs. controls at the same time; # p < 0.05 vs. diclofenac at the same time; ## p < 0.01 vs. diclofenac at the same time.
Figure 5
Figure 5
Effect of a single application of fucoidan from C. crinita (25 and 50 mg/kg bw) on serum levels of the pro-inflammatory cytokines IL-1β (panel A), TNF-α (panel B), and Il-6 (panel C) in LPS-induced systemic inflammation in rats. * p < 0.05 vs. same cytokine controls; ** p < 0.01 vs. same cytokine controls; *** p < 0.001 vs. same cytokine controls.
Figure 6
Figure 6
Effect of a single application of dexamethasone (0.2 mg/kg bw) and fucoidan from C. crinita (25 mg/kg bw) on the levels of TNF-α in the peritoneal fluid of rats with carrageenan-induced peritonitis. ** p < 0.01 vs. saline-treated controls.
Figure 7
Figure 7
Effects of a single dose of fucoidan from C. crinita (25 and 50 mg/kg bw) on serum levels of IL-10 in rats with LPS-induced systemic inflammation.
Figure 8
Figure 8
Macrosopic identification of Cystoseira crinita (Desf.) Bory.
Figure 9
Figure 9
Extraction process of fucoidan from C. crinita.
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Grants and funding

This research was funded by Medical University–Plovdiv through the Doctoral and Postdoctoral project-02/2019. The APC was funded by the same institution.

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