Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Mar;81(1):117-126.
doi: 10.1007/s12013-022-01114-z. Epub 2022 Nov 8.

Does Oxidative Stress Along with Dysbiosis Participate in the Pathogenesis of Asthma in the Obese?

Paulina Kleniewska  1 , Rafał Pawliczak  2
Affiliations

Does Oxidative Stress Along with Dysbiosis Participate in the Pathogenesis of Asthma in the Obese?

Paulina Kleniewska et al. Cell Biochem Biophys. 2023 Mar.

Abstract

The most important environmental factor that can play a key role in the development of asthma in the obese is overproduction of reactive oxygen species (ROS). The aim of the study was to examine changes in the concentration of oxidative stress parameters in the lungs, bronchoalveolar lavage (BAL) fluid and blood of mice in models of asthma or/and obesity caused by high-fat diet (HFD). The concentrations of 4-HNE and isoprostanes in the lungs of the animals were measured. BAL fluid levels of hydrogen peroxide were marked. Additionally, thiobarbituric acid reactive substances (TBARS) and ferric reducing ability of plasma (FRAP) were used as biomarkers of oxidative stress in the blood. Administration of lipoic acid (LA), a probiotic with standard-fat diet (SFD, 10% fat) and low-fat diet (LFD, 5% fat) significantly decreased the concentration of 4-HNE as compared to the OVA (ovalbumin) + HFD group (p < 0.05). Treatment with low-fat diet or LFD in combination with apocynin insignificantly decreased H2O2 values as compared to the OVA + HFD group. Supplementation of probiotic with SFD and LFD significantly decreased the concentration of TBARS as compared to the OVA + SFD and saline + HDF groups (p < 0.05). Significantly lower concentrations of TBARS were also observed in the LA plus LFD group (p < 0.05) as compared to the OVA + HFD group. Low-fat diet with probiotic significantly increased the concentration of FRAP as compared to the obese mice (p = 0.017). Treatment with LFD in combination with LA significantly increased FRAP values as compared to the obese and obese asthmatic mice (p < 0.001).

Keywords: Biomarkers; Oxidative stress; Reactive oxygen species.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
4-HNE levels in all the groups of animals. Data are shown as mean ± SD. a 4-HNE values [µg/ml] in the non-treatment groups (respectively: standard-fat diet group—control, standard-fat diet group with ovalbumin, high-fat diet group and high-fat diet group with ovalbumin); b 4-HNE values in the treatment groups (respectively: administration of apocynin—15 mg/kg with standard-fat diet; supplementation of low-fat diet alone; administration of apocynin—15 mg/kg with low-fat diet; supplementation of probiotic with low-fat diet; administration of lipoic acid—100 mg/kg with low-fat diet; supplementation of probiotic with standard-fat diet); c 4-HNE values [µg/ml] in all the groups of animals; #p < 0.05 vs. the 0.9% NaCl + SFD group; *p < 0.05 vs. the OVA + HFD group (Dunn’s method)
Fig. 2
Fig. 2
8-isoprostane levels in all the groups of animals. Data are shown as mean ± SD. a 8-isoprostane values [pg/ml] in the non-treatment groups (respectively: standard-fat diet group—control, standard-fat diet group with ovalbumin, high-fat diet group and high-fat diet group with ovalbumin); b 8-isoprostane values in the treatment groups (respectively: administration of apocynin—15 mg/kg with standard-fat diet; supplementation of low-fat diet alone; administration of apocynin—15 mg/kg with low-fat diet; supplementation of probiotic with low-fat diet; administration of lipoic acid—100 mg/kg with low-fat diet; supplementation of probiotic with standard-fat diet); c 8-isoprostane values [pg/ml] in all the groups of animals; #p < 0.05 vs. the 0.9% NaCl + SFD group (Dunn’s method)
Fig. 3
Fig. 3
H2O2 levels in all the groups of animals. Data are shown as mean ± SD. a H2O2 values [µmol] in the non-treatment groups (respectively: standard-fat diet group—control, standard-fat diet group with ovalbumin, high-fat diet group and high-fat diet group with ovalbumin); b H2O2 values in the treatment groups (respectively: administration of apocynin—15 mg/kg with standard-fat diet; supplementation of a low-fat diet alone; administration of apocynin—15 mg/kg with low-fat diet; supplementation of probiotic with low-fat-diet; administration of lipoic acid—100 mg/kg with low-fat diet; supplementation of probiotic with standard-fat diet); c H2O2 values [µmol] in all the groups of animals; #p < 0.05 vs. the 0.9% NaCl + SFD group (Dunn’s method)
Fig. 4
Fig. 4
TBARS levels in all the groups of animals. Data are shown as mean ± SD. a TBARS values [µmol] in the non-treatment groups (respectively: standard-fat diet group—control, standard-fat diet group with ovalbumin, high-fat diet group and high-fat diet group with ovalbumin); b TBARS values [µmol] in the treatment groups (respectively: administration of apocynin—15 mg/kg with standard-fat diet; supplementation of a low-fat diet alone; administration of apocynin—15 mg/kg with low-fat diet; supplementation of probiotic with low-fat diet; administration of lipoic acid—100 mg/kg with low-fat-diet; supplementation of probiotic with standard-fat-diet); c Final TBARS values [µmol] in all the groups of animals; #p < 0.05; vs. the 0.9% NaCl + HFD group; *p < 0.05 vs. the OVA + SFD group; &p < 0.05 vs. the OVA + HFD group (Dunn’s method)
Fig. 5
Fig. 5
FRAP levels in all the groups of animals. Data are shown as mean ± SD. a FRAP values [µmol] in the non-treatment groups (respectively: standard-fat diet group—control, standard-fat diet group with ovalbumin, high-fat diet group and high-fat diet group with ovalbumin); b FRAP values [µmol] in the treatment groups (respectively: administration of apocynin—15 mg/kg with standard-fat diet; supplementation of a low-fat diet alone; administration of apocynin—15 mg/kg with low-fat diet; supplementation of probiotic with low-fat diet; administration of lipoic acid—100 mg/kg with low-fat diet; supplementation of probiotic with standard-fat-diet); c Final FRAP values [µmol] in all the groups of animals; *p < 0.01; ‘p = 0.006; /p = 0.017 vs. the 0.9% NaCl + HFD and OVA + HFD groups; #p = 0.005; &p = 0.018; ^p = 0.033 vs. OVA + HFD (Tukey test)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bartosz G. Reactive oxygen species: Destroyers or messengers? Biochemical Pharmacology. 2009;77(8):1303–1315. doi: 10.1016/j.bcp.2008.11.009. - DOI - PubMed
    1. Lushchak VI. Free radicals, reactive oxygen species, oxidative stress and its classification. Chemico-Biological Interactions. 2014;224C(Oct):164–175. doi: 10.1016/j.cbi.2014.10.016. - DOI - PubMed
    1. Apel K, Hirt H. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology. 2004;55:373–399. doi: 10.1146/annurev.arplant.55.031903.141701. - DOI - PubMed
    1. Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: Properties, sources, targets, and their implication in various diseases. Indian Journal of Clinical Biochemistry. 2015;30(1):11–26. doi: 10.1007/s12291-014-0446-0. - DOI - PMC - PubMed
    1. Valko M, Leibfritz D, Moncol J, Cronin TDM, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology. 2007;39:44–84. doi: 10.1016/j.biocel.2006.07.001. - DOI - PubMed

Substances