A New HPLC-MS/MS Method for the Simultaneous Determination of Quercetin and Its Derivatives in Green Coffee Beans

doi:10.3390/foods11193033

. 2022 Sep 30;11(19):3033.

doi: 10.3390/foods11193033.

A New HPLC-MS/MS Method for the Simultaneous Determination of Quercetin and Its Derivatives in Green Coffee Beans

Affiliations

¹ School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
² Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
³ Illycaffè S.p.A., Via Flavia 110, 34147 Trieste, Italy.

PMID: 36230109
PMCID: PMC9563038
DOI: 10.3390/foods11193033

A New HPLC-MS/MS Method for the Simultaneous Determination of Quercetin and Its Derivatives in Green Coffee Beans

Ahmed M Mustafa et al. Foods. 2022.

. 2022 Sep 30;11(19):3033.

doi: 10.3390/foods11193033.

Authors

Affiliations

¹ School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
² Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
³ Illycaffè S.p.A., Via Flavia 110, 34147 Trieste, Italy.

PMID: 36230109
PMCID: PMC9563038
DOI: 10.3390/foods11193033

Abstract

Green coffee (Coffee arabica and Coffee robusta) is one of the most commonly traded goods globally. Their beans are enriched with polyphenols and numerous health benefits are associated with their consumption. The main aim of this work was to develop a new and fast analytical HPLC-MS/MS method to simultaneously determine six flavonoid polyphenolic compounds (quercetin, rutin, isorhamnetin, quercetin-3-glucouronide, hyperoside, and quercitrin) in 22 green coffee samples from six different geographical origins (Ethiopia, Brazil, Guatemala, Nicaragua, India and Colombia). In addition, by adjusting pH, temperature, solvent type, and extraction duration, several extraction methods such as acidic and alkaline hydrolysis, and extraction without hydrolysis were evaluated. The optimal extraction procedure in terms of recovery percentages (78.67-94.09%)was acidic hydrolysis at pH 2, extraction temperature of 60 °C, extraction solvent of 70% ethanol, and extraction duration of 1.5 h. Hyperoside (878-75 μg/kg) was the most abundant compound followed by quercitrin (408-38 μg/kg), quercetin (300-36 μg/kg), rutin (238-21 μg/kg), and quercetin-3-glucouronide (225-7 μg/kg), while isorhamnetin (34-3 μg/kg) showed the lowest amount. Overall, green coffee beans are rich in flavonoid polyphenolic compounds and could be used as part of a healthy diet.

Keywords: HPLC-MS/MS; extraction methods; flavonoids; green coffee; quercetin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
HPLC-MS/MS chromatograms of a standard mixture of the 6 analyzed quercetin derivatives (0.5 mg L⁻¹) plotted as overlapped (A) and separate (B) multiple reaction monitoring (MRM) transition of each analyte.

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References

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[1] Zengin G., Sinan K.I., Mahomoodally M.F., Angeloni S., Mustafa A.M., Vittori S., Maggi F., Caprioli G. Chemical composition, antioxidant and enzyme inhibitory properties of different extracts obtained from spent coffee ground and coffee silverskin. Foods. 2020;9:713. doi: 10.3390/foods9060713. - DOI - PMC - PubMed

[2] Zengin G., Sinan K.I., Mahomoodally M.F., Angeloni S., Mustafa A.M., Vittori S., Maggi F., Caprioli G. Chemical composition, antioxidant and enzyme inhibitory properties of different extracts obtained from spent coffee ground and coffee silverskin. Foods. 2020;9:713. doi: 10.3390/foods9060713. - DOI - PMC - PubMed

[3] Angeloni S., Navarini L., Khamitova G., Maggi F., Sagratini G., Vittori S., Caprioli G. A new analytical method for the simultaneous quantification of isoflavones and lignans in 25 green coffee samples by HPLC-MS/MS. Food Chem. 2020;325:126924. doi: 10.1016/j.foodchem.2020.126924. - DOI - PubMed

[4] Angeloni S., Navarini L., Khamitova G., Maggi F., Sagratini G., Vittori S., Caprioli G. A new analytical method for the simultaneous quantification of isoflavones and lignans in 25 green coffee samples by HPLC-MS/MS. Food Chem. 2020;325:126924. doi: 10.1016/j.foodchem.2020.126924. - DOI - PubMed

[5] Belguidoum K., Amira-Guebailia H., Boulmokh Y., Houache O. HPLC coupled to UV–vis detection for quantitative determination of phenolic compounds and caffeine in different brands of coffee in the Algerian market. J. Taiwan Inst. Chem. Eng. 2014;45:1314–1320. doi: 10.1016/j.jtice.2014.03.014. - DOI

[6] Belguidoum K., Amira-Guebailia H., Boulmokh Y., Houache O. HPLC coupled to UV–vis detection for quantitative determination of phenolic compounds and caffeine in different brands of coffee in the Algerian market. J. Taiwan Inst. Chem. Eng. 2014;45:1314–1320. doi: 10.1016/j.jtice.2014.03.014. - DOI

[7] Pimpley V., Patil S., Srinivasan K., Desai N., Murthy P.S. The chemistry of chlorogenic acid from green coffee and its role in attenuation of obesity and diabetes. Prep. Biochem. Biotechnol. 2020;50:969–978. doi: 10.1080/10826068.2020.1786699. - DOI - PubMed

[8] Pimpley V., Patil S., Srinivasan K., Desai N., Murthy P.S. The chemistry of chlorogenic acid from green coffee and its role in attenuation of obesity and diabetes. Prep. Biochem. Biotechnol. 2020;50:969–978. doi: 10.1080/10826068.2020.1786699. - DOI - PubMed

[9] Ali A.M.A., Yagi S., Qahtan A.A., Alatar A.A., Angeloni S., Maggi F., Caprioli G., Abdel-Salam E.M., Sinan K.I., Zengin G. Evaluation of the chemical constituents, antioxidant and enzyme inhibitory activities of six Yemeni green coffee beans varieties. Food Biosci. 2022;46:101552.

[10] Ali A.M.A., Yagi S., Qahtan A.A., Alatar A.A., Angeloni S., Maggi F., Caprioli G., Abdel-Salam E.M., Sinan K.I., Zengin G. Evaluation of the chemical constituents, antioxidant and enzyme inhibitory activities of six Yemeni green coffee beans varieties. Food Biosci. 2022;46:101552.

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A New HPLC-MS/MS Method for the Simultaneous Determination of Quercetin and Its Derivatives in Green Coffee Beans

Affiliations

A New HPLC-MS/MS Method for the Simultaneous Determination of Quercetin and Its Derivatives in Green Coffee Beans

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

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Cited by

References

Grants and funding

LinkOut - more resources

Full Text Sources