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. 2022 Aug 26:13:990677.
doi: 10.3389/fgene.2022.990677. eCollection 2022.

Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn, Macrobrachium rosenbergii

Affiliations

Comparative transcriptome analysis of brain and gonad reveals reproduction-related miRNAs in the giant prawn, Macrobrachium rosenbergii

Jiao Xia et al. Front Genet. .

Abstract

Macrobrachium rosenbergii (M. rosenbergii), as a species of common prawn, is a delicacy that is consumed all over the world. By interacting with the target gene 3'-untranslated region (3'-UTR), microRNAs (miRNAs) regulate its expression and ultimately participate in the regulation of reproductive development. However, research focusing on miRNA regulation during gonadal development in M. rosenbergii received very little attention. To explore the association between miRNA and reproduction, we performed RNA sequencing (RNA-seq) on brain and gonad organs in male and female M. rosenbergii. A total of 494 miRNAs were obtained in RNA-seq, including 31 and 59 differentially expressed (DE) miRNAs in the brain and gonads, respectively. Furthermore, 9 DE miRNAs were randomly selected from the brain and gonads, and qRT-PCR was conducted to validate the results of RNA-seq. Interestingly, dpu-miR-133 was found to be substantially expressed in the male brain and testis but poorly expressed in the female brain, ovary, and other organs. Analysis of dpu-miR-133 by Targetscan and MiRanda predicted to target 5-HT1. Furthermore, the dual-luciferase reporter assay manifested that dpu-miR-133 can combine with 5-HT1. Overall, our research work provides basic data for further study on the miRNA-mediated regulation of brain, gonad, and reproductive development of study M. rosenbergii.

Keywords: 5-HT1; MiR-133; RNA-seq; brain; gonad; macrobrachium rosenbergii; miRNA; reproduction.

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Conflict of interest statement

BL was empolyed by the company Huzhou Fengshengwan Aquatic Seed Industry Co. Ltd. The remaining authors declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Identification of miRNAs. (A) The distribution of miRNA length from the M. rosenbergii. (B) Statistic of differently expressed miRNA. (C) Volcanic map of M. rosenbergii differences in the brain. (D) Volcanic map of M. rosenbergii differences in the gonad. MB: male brain; FB: female brain; T: testis; O: ovary.
FIGURE 2
FIGURE 2
Top 30 significantly enriched KEGG pathways of putative target genes between the female and male macrobrachium rosenbergii in the brain (A) and gonad (B). Significant enrichment of the KEGG pathway (p < 0.05). The color represents the size of the p-value and the point represents pathway. The redder the color, the smaller the p-value, the larger the number of points, and the more enriched genes. MB: male brain; FB: female brain; T: testis; O: ovary.
FIGURE 3
FIGURE 3
Top 30 GO terms of unique genes targeted by DEMs from the female and male macrobrachium rosenbergii in the brain (A) and gonad (B). GO analysis falls into three categories: biological processes, molecular functions, and cellular components. MB: male brain; FB: female brain; T: testis; O: ovary.
FIGURE 4
FIGURE 4
The expression of DEMs in the brain of M. rosenbergii. The endogenous reference gene was U6, and its expression was determined by comparative CT (ΔΔCT) methods. T: testis; O: ovary; M: muscle; L: liver; G: gill; MB: male brain; FB: female brain. *: 0.01 < p < 0.05; **: 0.001 < p < 0.01; ***: p < 0.001.
FIGURE 5
FIGURE 5
The expression of DEMs in the gonad of M. rosenbergii. The endogenous reference gene was U6, and its expression was determined by comparative CT (ΔΔCT) methods. M: muscle; B: brain; L: liver; G gill; I: intestines; O: ovary; T: testis. *: 0.01 < p < 0.05; **: 0.001 < p < 0.01; ***: p < 0.001.
FIGURE 6
FIGURE 6
Double luciferase reporter gene analysis of miR-133 and 5-HT1. (A). The binding site of miR-133 to 5-HT1 3’-UTR. (B). Relative luciferase activity in HEK293 cells. GraphPad Prism 7 draws a histogram. The means ± standard deviations of the three independent experiments are shown in the data. *p < 0.05.

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References

    1. Ahmed K., LaPierre M. P., Gasser E., Denzler R., Yang Y., Rulicke T., et al. (2017). Loss of microRNA-7a2 induces hypogonadotropic hypogonadism and infertility. J. Clin. Investig. 127 (3), 1061–1074. 10.1172/JCI90031 - DOI - PMC - PubMed
    1. Bartel D. P. (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 116 (2), 281–297. 10.1016/s0092-8674(04)00045-5 - DOI - PubMed
    1. Benmansour S., Privratsky A. A., Adeniji O. S., Frazer A. (2014). Signaling mechanisms involved in the acute effects of estradiol on 5-HT clearance. Int. J. Neuropsychopharmacol. 17 (5), 765–777. 10.1017/S146114571300165X - DOI - PMC - PubMed
    1. Betel D., Wilson M., Gabow A., Marks D. S., Sander C. (2008). The microRNA.org resource: targets and expression. Nucleic Acids Res. 36, D149–D153. 10.1093/nar/gkm995 - DOI - PMC - PubMed
    1. Bizuayehu T. T., Babiak J., Norberg B., Fernandes J. M., Johansen S. D., Babiak I. (2012). Sex-biased miRNA expression in Atlantic halibut (Hippoglossus hippoglossus) brain and gonads. Sex. Dev. 6 (5), 257–266. 10.1159/000341378 - DOI - PubMed

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