Addressing artifacts of colorimetric anticancer assays for plant-based drug development

doi:10.1007/s12032-022-01791-z

Review

. 2022 Sep 7;39(12):198.

doi: 10.1007/s12032-022-01791-z.

Addressing artifacts of colorimetric anticancer assays for plant-based drug development

Salma Batool¹, Seejal Javaid², Hira Javed², Laiba Asim², Izzah Shahid³, Mishal Khan⁴, Amna Muhammad⁵

Affiliations

¹ Department of Biochemistry, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan. salma.batool@ucp.edu.pk.
² Department of Biochemistry, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
³ Department of Biotechnology, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
⁴ Institute of Systems Engineering (IMSE), Heidelberg University, Heidelberg, Germany.
⁵ Department of Biochemistry, Pir Mehar Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.

PMID: 36071299
DOI: 10.1007/s12032-022-01791-z

Review

Addressing artifacts of colorimetric anticancer assays for plant-based drug development

Salma Batool et al. Med Oncol. 2022.

. 2022 Sep 7;39(12):198.

doi: 10.1007/s12032-022-01791-z.

Authors

Salma Batool¹, Seejal Javaid², Hira Javed², Laiba Asim², Izzah Shahid³, Mishal Khan⁴, Amna Muhammad⁵

Affiliations

¹ Department of Biochemistry, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan. salma.batool@ucp.edu.pk.
² Department of Biochemistry, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
³ Department of Biotechnology, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
⁴ Institute of Systems Engineering (IMSE), Heidelberg University, Heidelberg, Germany.
⁵ Department of Biochemistry, Pir Mehar Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.

PMID: 36071299
DOI: 10.1007/s12032-022-01791-z

Abstract

Cancer has become the silent killer in less-developed countries and the most significant cause of morbidity worldwide. The accessible and frequently used treatments include surgery, radiotherapy, chemotherapy, and immunotherapy. Chemotherapeutic drugs traditionally involve using plant-based medications either in the form of isolated compounds or as scaffolds for synthetic drugs. To launch a drug in the market, it has to pass through several intricate steps. The multidrug resistance in cancers calls for novel drug discovery and development. Every year anticancer potential of several plant-based compounds and extracts is reported but only a few advances to clinical trials. The false-positive or negative results impact the progress of the cell-based anticancer assays. There are several cell-based assays but the widely used include MTT, MTS, and XTT. In this article, we have discussed various pitfalls and workable solutions.

Keywords: Anticancer; Anticancer drugs; Colorimetric assays; Drug development; MTS; MTT; Pitfalls or solutions; XTT.

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References

1. Ediriweera MK, Tennekoon KH, Samarakoon SR. In vitro assays and techniques utilized in anticancer drug discovery. Appl Toxicol. 2019;39(1):38–71. - DOI
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1. Dube PN, Sakle NS, Dhawale SA, More SA, Mokale SN. Synthesis, biological investigation and docking study of novel chromen derivatives as anti-cancer agents. Anti-Cancer Agents Med Chem. 2019;19(9):1150–60. - DOI

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[1] Ediriweera MK, Tennekoon KH, Samarakoon SR. In vitro assays and techniques utilized in anticancer drug discovery. Appl Toxicol. 2019;39(1):38–71. - DOI

[2] Ediriweera MK, Tennekoon KH, Samarakoon SR. In vitro assays and techniques utilized in anticancer drug discovery. Appl Toxicol. 2019;39(1):38–71. - DOI

[3] Rayan A, Raiyn J, Falah M. Nature is the best source of anticancer drugs: indexing natural products for their anticancer bioactivity. J PLoS one. 2017;12(11):187925. - DOI

[4] Rayan A, Raiyn J, Falah M. Nature is the best source of anticancer drugs: indexing natural products for their anticancer bioactivity. J PLoS one. 2017;12(11):187925. - DOI

[5] Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA. 2021;71(3):209–49. - PubMed

[6] Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA. 2021;71(3):209–49. - PubMed

[7] Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. J CA. 2015;65(2):87–108. - PubMed

[8] Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. J CA. 2015;65(2):87–108. - PubMed

[9] Dube PN, Sakle NS, Dhawale SA, More SA, Mokale SN. Synthesis, biological investigation and docking study of novel chromen derivatives as anti-cancer agents. Anti-Cancer Agents Med Chem. 2019;19(9):1150–60. - DOI

[10] Dube PN, Sakle NS, Dhawale SA, More SA, Mokale SN. Synthesis, biological investigation and docking study of novel chromen derivatives as anti-cancer agents. Anti-Cancer Agents Med Chem. 2019;19(9):1150–60. - DOI

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Addressing artifacts of colorimetric anticancer assays for plant-based drug development

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Addressing artifacts of colorimetric anticancer assays for plant-based drug development

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