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. 2022 Aug 19:15:2535-2543.
doi: 10.2147/DMSO.S369012. eCollection 2022.

Association of lncRNA LINC01173 Expression with Vitamin-D and Vitamin B12 Level Among Type 2 Diabetes Patients

Alanoud Aladel #  1 , Amit K Verma #  2 , Sadaf Dabeer  3 , Irfan Ahmad  4 , Mohammad Y Alshahrani  4 , Mohammad S AboHassan  4 , Mohammad Idreesh Khan #  5 , Malak Ghazi Almutairi  6 , Mirza Masroor Ali Beg  7   8
Affiliations

Association of lncRNA LINC01173 Expression with Vitamin-D and Vitamin B12 Level Among Type 2 Diabetes Patients

Alanoud Aladel et al. Diabetes Metab Syndr Obes. .

Abstract

Background: Type 2 diabetes mellitus (T2DM) has risen to become the world's most serious public health problem in recent years, and the role of long noncoding RNAs (lncRNAs) in the onset and progression of T2DM, as well as special attention to vitamins, has gotten a lot of attention recently.

Methods: The aim of the study was to analyze lncRNA LINC01173 expression along with assessment of vitamin-D and B12 among the T2DM cases. Quantitative RT-PCR was used to analyze the expression of lncRNA LINC01173. Vitamin-D and B12 were analyzed by chemiluminescence-based assay.

Results: The present study observed that the T2DM cases had 6.67-fold increased lncRNA LINC01173 expression compared to healthy controls. Expression of lncRNA LINC01173 was found to be associated with hypertension (p=0.03), wound healing (p=0.04), and blurred vision (p<0.0001). It was observed that the T2DM cases with vitamin-D deficiency had a significant association with fasting glucose level (p=0.01) and HbA1C level (p=0.01) among the T2DM cases. The association of lncRNA LINC01173 with vitamin-D was analyzed and it was observed that the vitamin-D deficient cases had higher lncRNA LINC01173 expression compared to insufficient T2DM cases (p=0.01) and sufficient T2DM cases (p=0.0006). It was also observed that the T2DM cases with smoking had a 8.33-fold lncRNA LINC01173 expression while non-smokers had a 5.43-fold lncRNA LINC01173 expression (p<0.0001).

Conclusion: The study concluded that the increased lncRNA LINC01173 expression was observed to be linked with alteration in vitamin-D level and smoking habit. Altered expression of lncRNA LINC01173 expression was linked with fasting glucose and HbA1C alteration. Collectively, lncRNA LINC01173 expression, vitamin-D alteration, as well as smoking habit may cause the disease severity and increase the pathogenesis of disease.

Keywords: diabetes; long noncoding RNA; vitamin-B12; vitamin-D.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Association of lncRNA LINC01173 expression with vitamin-D and B12. (A) lncRNA LINC01173 expression with respect to vitamin-D deficiency, insufficiency and sufficiency. (B) lncRNA LINC01173 expression with respect to B12 deficient and normal level.
Figure 2
Figure 2
lncRNA LINC01173 expression and its association with smoking and nonsmoking habit of T2DM cases.
See this image and copyright information in PMC

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