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. 2022:2540:219-237.
doi: 10.1007/978-1-0716-2541-5_10.

Studying Protein Function Using Nanobodies and Other Protein Binders in Drosophila

Katarzyna Lepeta #  1 , Milena Bauer #  1 , Gustavo Aguilar #  1 , M Alessandra Vigano  1 , Shinya Matsuda  1 , Markus Affolter  2
Affiliations

Studying Protein Function Using Nanobodies and Other Protein Binders in Drosophila

Katarzyna Lepeta et al. Methods Mol Biol. 2022.

Abstract

The direct manipulation of proteins by nanobodies and other protein binders has become an additional and valuable approach to investigate development and homeostasis in Drosophila. In contrast to other techniques, that indirectly interfere with proteins via their nucleic acids (CRISPR, RNAi, etc.), protein binders permit direct and acute protein manipulation. Since the first use of a nanobody in Drosophila a decade ago, many different applications exploiting protein binders have been introduced. Most of these applications use nanobodies against GFP to regulate GFP fusion proteins. In order to exert specific protein manipulations, protein binders are linked to domains that confer them precise biochemical functions. Here, we reflect on the use of tools based on protein binders in Drosophila. We describe their key features and provide an overview of the available reagents. Finally, we briefly explore the future avenues that protein binders might open up and thus further contribute to better understand development and homeostasis of multicellular organisms.

Keywords: DARPins; Development; Drosophila; GFP; Nanobodies; Protein Binders.

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