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. 1987 Feb 15;242(1):55-9.
doi: 10.1042/bj2420055.

Heparin-induced release of extracellular superoxide dismutase to human blood plasma

K Karlsson, S L Marklund

Heparin-induced release of extracellular superoxide dismutase to human blood plasma

K Karlsson et al. Biochem J. .

Abstract

Extracellular superoxide dismutase (SOD) has previously been shown to be the major SOD isoenzyme in extracellular fluids. Upon chromatography on heparin-Sepharose it was separated into three fractions: A, without affinity; B, with intermediate affinity; and C, with relatively strong heparin affinity. Intravenous injection of heparin leads to a prompt increase in plasma extracellular-superoxide-dismutase (EC-SOD) activity. Heparin induces no release of EC-SOD from blood cells, nor does it activate EC-SOD in plasma, indicating that the source of the released enzyme is the endothelial-cell surfaces. No distinct saturation could be demonstrated in a dose-response curve up to 200 i.u. of heparin per kg body weight, showing that the releasing potency of heparin is lower for EC-SOD than for previously investigated heparin-released factors. Chromatography of human plasma on heparin-Sepharose shows nearly equal amounts of EC-SOD fractions A, B and C. Heparin induces specifically the release of fraction C. The findings point to the existence of an equilibrium of EC-SOD fraction C between the plasma phase and endothelial-cell surfaces. The major part of EC-SOD in the vasculature seems to be located on endothelial-cell surfaces.

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References

    1. Acta Med Scand. 1966 Nov;180(5):533-6 - PubMed
    1. J Lipid Res. 1973 May;14(3):286-95 - PubMed
    1. J Biol Chem. 1976 Dec 10;251(23):7504-7 - PubMed
    1. Thromb Res. 1978 May;12(5):851-61 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1159-63 - PubMed

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