In vivo therapeutic efficacy of Curcuma longa extract loaded ethosomes on wound healing
- PMID: 35796857
- DOI: 10.1007/s11259-022-09952-1
In vivo therapeutic efficacy of Curcuma longa extract loaded ethosomes on wound healing
Abstract
Since ancient times, medicinal plants are widely accepted to promote the health and wellness of animals and mankind. The medicinal plant-based therapies have limitations of delayed onset of action, inconsistent absorption, low bioavailability, oxidation, and poor solubility. The encapsulation studies suggested improved efficacy. Therefore, the present study attempts to evaluate the efficacy of Curcuma longa extracts encapsulated in Ethosome on wound healing model compared to crude extract. The Curcuma longa extract swere prepared by cold percolation method and total curcuminoid content was determined by Reverse phase-HPLC. Three Ethosomal suspensions (ETS1, ETS2, and ETS3) were prepared and characterized for particle distribution, morphology, and absorption spectrum by Zetasizer, Scanning Electron Microscopy, and FTIR respectively. The Ethosomal suspension with the highest entrapment efficiency was applied topically at a varying concentrations (0.25, 0.5, and 1 g/cm2) on the surgically created wounds in rats. The efficacy of wound healing was evaluated by clinical observation, macroscopic evaluation of granulation tissue, colour digital image processing, and histology. The methanolic extract of Curcuma longa showed better antibacterial potential than ethanolic and aqueous. The total Curcuminoid content in the Curcuma longa rhizome was 4.03%. The size, PDI, zeta potential, and viscosity of Ethosomal suspension ranged from 34.8 to 371 nm, 0.236-1.178, 15.6-36.8mV, and 0.8460-0.8510, respectively. The ETS3 was found the most optimum combination with the highest entrapment efficiency and the topical application at a dose rate of 0.5 g/cm2 and 1.0 g/cm2 resulted in comparable wound contracture, pain score, histopathological score as compared to control groups.It was concluded that the Curcuma longa encapsulation in Ethosome resulted in improved wound appearance, granulation tissue score, and appearance with a shortened period of wound resolution at the cellular level as compared to crude extract.
Keywords: Curcuma longa; Encapsulation; Ethosome; Herbal; Rats; Wound healing.
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
Similar articles
-
Topical vesicular formulations of Curcuma longa extract on recuperating the ultraviolet radiation-damaged skin.J Cosmet Dermatol. 2011 Dec;10(4):260-5. doi: 10.1111/j.1473-2165.2011.00586.x. J Cosmet Dermatol. 2011. PMID: 22151933
-
Exploring the role of curcumin containing ethanolic extract obtained from Curcuma longa (rhizomes) against retardation of wound healing process by aspirin.Indian J Pharmacol. 2015 Mar-Apr;47(2):160-6. doi: 10.4103/0253-7613.153422. Indian J Pharmacol. 2015. PMID: 25878374 Free PMC article.
-
REPORT-Effect of topical Curcuma longa extract gel on incision wound angiogenesis in Balb/C mice.Pak J Pharm Sci. 2021 May;34(3):1023-1029. Pak J Pharm Sci. 2021. PMID: 34602428
-
Curcuma longa aqueous extract: A potential solution for the prevention of corneal scarring as a result of pterygium surgical excision (Review).Int J Mol Med. 2020 Dec;46(6):1951-1957. doi: 10.3892/ijmm.2020.4738. Epub 2020 Sep 25. Int J Mol Med. 2020. PMID: 33125502 Review.
-
Effectiveness of Curcuma longa extract versus placebo for the treatment of knee osteoarthritis: A systematic review and meta-analysis of randomized controlled trials.Phytother Res. 2021 Nov;35(11):5921-5935. doi: 10.1002/ptr.7204. Epub 2021 Jul 2. Phytother Res. 2021. PMID: 34216044 Review.
Cited by
-
Dimethyl Fumarate-Loaded Transethosomes: A Formulative Study and Preliminary Ex Vivo and In Vivo Evaluation.Int J Mol Sci. 2022 Aug 6;23(15):8756. doi: 10.3390/ijms23158756. Int J Mol Sci. 2022. PMID: 35955900 Free PMC article.
-
Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2.Saudi Pharm J. 2024 Jul;32(7):102110. doi: 10.1016/j.jsps.2024.102110. Epub 2024 May 17. Saudi Pharm J. 2024. PMID: 38817820 Free PMC article.
-
The wound healing effect of botanicals and pure natural substances used in in vivo models.Inflammopharmacology. 2023 Apr;31(2):755-772. doi: 10.1007/s10787-023-01157-5. Epub 2023 Feb 22. Inflammopharmacology. 2023. PMID: 36811778 Free PMC article. Review.
References
-
- Ajige L, Sunday T, Elkanah F et al (2017) Comparative study of antibacterial effects of Curcuma longa Linn. and Zingiber officinale Rosc. Rhizomes. Microbiol Res J Int 18:1–8. https://doi.org/10.9734/mrji/2017/31551 - DOI
-
- Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R (2014) Curcumin as a wound healing agent. Life Sci 116:1–7 - PubMed
-
- Alemi A, Zavar Reza J, Haghiralsadat F et al (2018) Paclitaxel and curcumin coadministration in novel cationic PEGylated niosomal formulations exhibit enhanced synergistic antitumor efficacy. J Nanobiotechnol 16:1–20. https://doi.org/10.1186/s12951-018-0351-4 - DOI
-
- Antony B, Benny M, Rao SB (2005) Enhancing the absorption of curcuminoids. Spice India 23:26
-
- Asai A, Miyazawa T (2000) Occurrence of orally administered curcuminoid as glucuronide and glucuronide/sulfate conjugates in rat plasma. Life Sci 67:2785–2793. https://doi.org/10.1016/S0024-3205(00)00868-7 - DOI - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous