Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec;46(4):1033-1049.
doi: 10.1007/s11259-022-09952-1. Epub 2022 Jul 7.

In vivo therapeutic efficacy of Curcuma longa extract loaded ethosomes on wound healing

Affiliations

In vivo therapeutic efficacy of Curcuma longa extract loaded ethosomes on wound healing

Sumit Kumar et al. Vet Res Commun. 2022 Dec.

Abstract

Since ancient times, medicinal plants are widely accepted to promote the health and wellness of animals and mankind. The medicinal plant-based therapies have limitations of delayed onset of action, inconsistent absorption, low bioavailability, oxidation, and poor solubility. The encapsulation studies suggested improved efficacy. Therefore, the present study attempts to evaluate the efficacy of Curcuma longa extracts encapsulated in Ethosome on wound healing model compared to crude extract. The Curcuma longa extract swere prepared by cold percolation method and total curcuminoid content was determined by Reverse phase-HPLC. Three Ethosomal suspensions (ETS1, ETS2, and ETS3) were prepared and characterized for particle distribution, morphology, and absorption spectrum by Zetasizer, Scanning Electron Microscopy, and FTIR respectively. The Ethosomal suspension with the highest entrapment efficiency was applied topically at a varying concentrations (0.25, 0.5, and 1 g/cm2) on the surgically created wounds in rats. The efficacy of wound healing was evaluated by clinical observation, macroscopic evaluation of granulation tissue, colour digital image processing, and histology. The methanolic extract of Curcuma longa showed better antibacterial potential than ethanolic and aqueous. The total Curcuminoid content in the Curcuma longa rhizome was 4.03%. The size, PDI, zeta potential, and viscosity of Ethosomal suspension ranged from 34.8 to 371 nm, 0.236-1.178, 15.6-36.8mV, and 0.8460-0.8510, respectively. The ETS3 was found the most optimum combination with the highest entrapment efficiency and the topical application at a dose rate of 0.5 g/cm2 and 1.0 g/cm2 resulted in comparable wound contracture, pain score, histopathological score as compared to control groups.It was concluded that the Curcuma longa encapsulation in Ethosome resulted in improved wound appearance, granulation tissue score, and appearance with a shortened period of wound resolution at the cellular level as compared to crude extract.

Keywords: Curcuma longa; Encapsulation; Ethosome; Herbal; Rats; Wound healing.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Ajige L, Sunday T, Elkanah F et al (2017) Comparative study of antibacterial effects of Curcuma longa Linn. and Zingiber officinale Rosc. Rhizomes. Microbiol Res J Int 18:1–8. https://doi.org/10.9734/mrji/2017/31551 - DOI
    1. Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R (2014) Curcumin as a wound healing agent. Life Sci 116:1–7 - PubMed
    1. Alemi A, Zavar Reza J, Haghiralsadat F et al (2018) Paclitaxel and curcumin coadministration in novel cationic PEGylated niosomal formulations exhibit enhanced synergistic antitumor efficacy. J Nanobiotechnol 16:1–20. https://doi.org/10.1186/s12951-018-0351-4 - DOI
    1. Antony B, Benny M, Rao SB (2005) Enhancing the absorption of curcuminoids. Spice India 23:26
    1. Asai A, Miyazawa T (2000) Occurrence of orally administered curcuminoid as glucuronide and glucuronide/sulfate conjugates in rat plasma. Life Sci 67:2785–2793. https://doi.org/10.1016/S0024-3205(00)00868-7 - DOI - PubMed

LinkOut - more resources