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. 2022 Jun 9;11(6):1135.
doi: 10.3390/antiox11061135.

Polyphenols and IUGR Pregnancies: Effects of the Antioxidant Hydroxytyrosol on the Hippocampus Proteome in a Porcine Model

Natalia Yeste  1 , Jorge Pérez-Valle  1 , Marta Vázquez-Gómez  2   3 , Consolación García-Contreras  4   5 , Antonio González-Bulnes  2   5   6 , Anna Bassols  1
Affiliations

Polyphenols and IUGR Pregnancies: Effects of the Antioxidant Hydroxytyrosol on the Hippocampus Proteome in a Porcine Model

Natalia Yeste et al. Antioxidants (Basel). .

Abstract

Supplementation of a mother's diet with antioxidants such as hydroxytyrosol (HTX) has been proposed to ameliorate the adverse phenotypes of foetuses affected by intrauterine growth restriction (IUGR). Our previous studies showed, in a porcine model of IUGR, an effect of maternal HTX supplementation on the neurotransmitter profile of several brain areas and the morphology of the hippocampus in 100 days old foetuses. The present study analyzed the impact of maternal HTX supplementation on the hippocampus proteome at this foetal age by TMT10plex labelling. Eleven differentially abundant proteins were identified by comparing both conditions, and eight of them downregulated and three upregulated in the HTX-treated group. The downregulated proteins were mainly involved in protein synthesis and RNA metabolism and may explain the differences in neuron differentiation in the HTX-treated group. The upregulated proteins were related to cell detoxification and could represent a potential mechanism to explain the neuroprotective effect of HTX.

Keywords: TMT labelling; brain; hippocampus; hydroxytyrosol; intrauterine growth restriction; pig; proteome.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
GO-slim categories of DAPs in the comparison between the Ctrl and HTX groups.
Figure 2
Figure 2
Graphic representation by STRING of the interactions of the identified DAPs. The nodes represent the identified proteins, and the lines represent associations from the different sources: blue from databases, pink from experimental determinations, dark green from predictions due to closeness of genes, yellow from to appearance in documents, and black from co-expression.
See this image and copyright information in PMC

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