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. 2022 Sep;32(5):1843-1849.
doi: 10.1007/s10895-022-02996-1. Epub 2022 Jun 22.

The Development of a 4-aminonaphthalimide-based Highly Selective Fluorescent Probe for Rapid Detection of HOCl

Xiaodan Zeng  1 , Jie Chen  2 , Shihua Yu  3 , Zhigang Liu  2 , Mingshuo Ma  2
Affiliations

The Development of a 4-aminonaphthalimide-based Highly Selective Fluorescent Probe for Rapid Detection of HOCl

Xiaodan Zeng et al. J Fluoresc. 2022 Sep.

Abstract

Recently, more and more evidence indicated that intracellular HOCl plays a crucial role in the regulation of inflammation and apoptosis, while excessive HOCl has an impact on human health problems. So, the development of methods for sensitive detection of HOCl is very vital to reveal its various physiological and pathological functions. In this paper, we have described a simple fluorescent probe for selective detection of HOCl, whereas for higher concentrations of other biological important substances, the probe almost does not respond. The experimental results show that the probe can quantitatively determine the range of 0-1 μM HOCl, the detection limit is 0.05 μM. In addition, the probe reacts quickly with HOCl (< 3 s), which is helpful to monitor HOCl in biological system because HOCl is highly reactive and short-lived. The ability of the probe to HOCl enables it to be used to track the HOCl levels in living systems.

Keywords: 4-aminonaphthalimide; Fluorescence imaging; Fluorescent probe; Hypochlorous acid.

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