The sequence of an embryonic myosin heavy chain gene and isolation of its corresponding cDNA
- PMID: 3571266
The sequence of an embryonic myosin heavy chain gene and isolation of its corresponding cDNA
Abstract
The complete sequence of an embryonic chicken myosin heavy chain has been determined. Introns and exons were identified by comparison with the corresponding cDNA. The cDNA contains 5,962 bases, of which 85 bases constitute the poly(A) tail. The cDNA represents the entire mRNA transcript, except for 90 bases at the 5'-coding terminus and 101 bases of the 5'-untranslated region. The gene's coding region is split by 37 introns; two additional introns split the 101 base pairs which make up the 5'-untranslated region. The complete gene is approximately 23,000 base pairs and encodes a protein whose molecular weight is 222,559 and consists of 1,940 amino acids. Analysis of the protein and comparison with other myosin sequences reveal that certain regions have been conserved; those amino acids which have been postulated to participate in the ATPase and actin-binding activities of the molecule are highly homologous. These comparisons have allowed the identification of isolated regions within the myosin heavy chain that appear to be essential for the molecule's function.
Similar articles
-
The complete sequence of the human beta-myosin heavy chain gene and a comparative analysis of its product.Genomics. 1990 Oct;8(2):194-206. doi: 10.1016/0888-7543(90)90272-v. Genomics. 1990. PMID: 2249844
-
Isolation of the chick myosin alkali light chain gene expressed in embryonic gizzard muscle and transitional expression of the light chain gene family in vivo.J Mol Biol. 1988 Dec 5;204(3):497-505. doi: 10.1016/0022-2836(88)90350-6. J Mol Biol. 1988. PMID: 3225843
-
Complete nucleotide sequence and deduced polypeptide sequence of a nonmuscle myosin heavy chain gene from Acanthamoeba: evidence of a hinge in the rodlike tail.J Cell Biol. 1987 Aug;105(2):913-25. doi: 10.1083/jcb.105.2.913. J Cell Biol. 1987. PMID: 3040773 Free PMC article.
-
Characterization of the myosin light-chain-2 gene of Drosophila melanogaster.Mol Cell Biol. 1985 Nov;5(11):3058-68. doi: 10.1128/mcb.5.11.3058-3068.1985. Mol Cell Biol. 1985. PMID: 3018498 Free PMC article.
-
Identification of two types of smooth muscle myosin heavy chain isoforms by cDNA cloning and immunoblot analysis.J Biol Chem. 1989 Jun 15;264(17):9734-7. J Biol Chem. 1989. PMID: 2722872
Cited by
-
Role of the COOH-terminal nonhelical tailpiece in the assembly of a vertebrate nonmuscle myosin rod.J Cell Biol. 1992 Sep;118(5):1085-95. doi: 10.1083/jcb.118.5.1085. J Cell Biol. 1992. PMID: 1512291 Free PMC article.
-
Expression and DNA sequence analysis of a human embryonic skeletal muscle myosin heavy chain gene.Nucleic Acids Res. 1989 Aug 11;17(15):6167-79. doi: 10.1093/nar/17.15.6167. Nucleic Acids Res. 1989. PMID: 2771643 Free PMC article.
-
Sexually dimorphic expression of a laryngeal-specific, androgen-regulated myosin heavy chain gene during Xenopus laevis development.Dev Biol. 1992 Dec;154(2):366-76. doi: 10.1016/0012-1606(92)90075-r. Dev Biol. 1992. PMID: 1426643 Free PMC article.
-
The myosin filament XV assembly: contributions of 195 residue segments of the myosin rod and the eight C-terminal residues.J Muscle Res Cell Motil. 1996 Oct;17(5):555-73. doi: 10.1007/BF00124355. J Muscle Res Cell Motil. 1996. PMID: 8906623
-
Thin filament remodeling in failing myocardium.Heart Fail Rev. 2005 Sep;10(3):199-209. doi: 10.1007/s10741-005-5250-8. Heart Fail Rev. 2005. PMID: 16416043 Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
