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. 2022 Apr 27;17(1):816-825.
doi: 10.1515/med-2022-0472. eCollection 2022.

Low expression of miR-27b in serum exosomes of non-small cell lung cancer facilitates its progression by affecting EGFR

Xiying Cao  1 , Weixiang Zhong  2 , Shaoming Guo  2 , Zuxiong Zhang  2 , Chunfa Xie  2
Affiliations

Low expression of miR-27b in serum exosomes of non-small cell lung cancer facilitates its progression by affecting EGFR

Xiying Cao et al. Open Med (Wars). .

Abstract

Non-small cell lung cancer (NSCLC) is a malignant tumor. Serum exosomal miR-27b is related to tumor diagnosis. We explored the roles of serum exosomal miR-27b in NSCLC. NSCLC patients were assigned to NSCLC-early/terminal groups, with healthy subjects as controls. miR-27b expression was assessed using reverse transcription-quantitative polymerase chain reaction, and its diagnostic efficiency was analyzed using the receiver operating characteristic curve. The correlation between serum exosomal miR-27b expression and tumor markers carcinoembryonic antigen 125 (CA125), carcinoembryonic antigen (CEA), and cytokeratin 19-soluble fragment (CYFRA21-1) was analyzed using the Pearson analysis. The downstream target genes were predicted. Epidermal growth factor receptor (EGFR) level was assessed using enzyme-linked immunosorbent assay. Correlations of miR-27b expression with serum EGFR level and CA125, CEA, and CYFRA21-1 levels were analyzed using the Pearson analysis. Serum exosomal miR-27b was diminished in NSCLC and was further decreased in the NSCLS-terminal group. The sensitivity of miR-27b < 0.8150 for NSCLC diagnosis was 76.64%, and the specificity was 83.33%. Serum exosomal miR-27b was negatively correlated with CA125, CEA, and CYFRA21-1. miR-27b targeted EGFR. Serum EGFR was raised in NSCLC and was further elevated in the NSCLS-terminal group. miR-27b expression was negatively correlated with EGFR level. EGFR level was positively correlated with CA125, CEA, and CYFRA21-1 levels. Collectively, low expression of miR-27b assisted NSCLC diagnosis, and miR-27b exerted effects on NSCLC through EGFR.

Keywords: CA125; CEA; CYFR21-1; EGFR; Pearson analysis; ROC curve; exosome; miR-27b; non-small cell lung cancer.

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Conflict of interest statement

Conflict of interest: None of the authors of this article have conflicts of interest.

Figures

Figure 1
Figure 1
Morphology of exosome under TEM (uranyl acetate staining): Bar = 500 nm.
Figure 2
Figure 2
The expression of miR-27b was diminished in serum exosomes of NSCLC patients. (a) The expression of serum miR-27b was detected using RT-qPCR. (b) The expression of serum exosomal miR-27b was detected using RT-qPCR. The data were expressed as mean ± standard deviation. One-way analysis of variance (ANOVA) was applied for comparison among groups, followed by Tukey’s multiple comparisons test. ***P < 0.001.
Figure 3
Figure 3
The ROC curve of miR-27b expression for NSCLC diagnosis: (a) The ROC curve of miR-27b for control and NSCLC diagnosis; (b) the ROC curve of miR-27b for relative NSCLC early/terminal; and the ROC analysis was employed for data analysis in panels a and b.
Figure 4
Figure 4
The correlation analysis between serum exosomal miR-27b and CA125, CEA, and CYFRA21-1 levels. (a) Pearson analysis was applied for correlation analysis between serum exosomal miR-27b expression in the NSCLC-early/NSCLC-terminal groups and CA125 level; (b) Pearson analysis was applied for correlation analysis between serum exosomal miR-27b expression in the NSCLC-early/NSCLC-terminal groups and CEA level; (c) Pearson analysis was applied for correlation analysis between serum exosomal miR-27b expression in the NSCLC-early/NSCLC-terminal groups and CYFRA21-1 level. The Pearson analysis was employed for data in panels a, b, and c.
Figure 5
Figure 5
miR-27b might affect NSCLC progression through EGFR. (a) The target binding sites between miR-27b and EGFR were predicted and determined using a database; (b) the level of EGFR was detected by ELISA; (c) the correlation between miR-27b and EGFR level in the serum was analyzed using the Pearson analysis; (d) Pearson analysis was applied for correlation analysis between serum EGFR level in the NSCLC-early/NSCLC-terminal groups and CA125, CEA, and CYFRA21-1 levels. One-way analysis of variance (ANOVA) was applied for comparison among groups in panel b, followed by Tukey’s multiple comparisons test. ***P < 0.001. Pearson analysis was used for data analysis in panels c and d.
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