Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Feb;115(2):247-53.
doi: 10.1093/genetics/115.2.247.

Mutations causing constitutive invertase synthesis in yeast: genetic interactions with snf mutations

L Neigeborn, M Carlson

Mutations causing constitutive invertase synthesis in yeast: genetic interactions with snf mutations

L Neigeborn et al. Genetics. 1987 Feb.

Abstract

We have selected 210 mutants able to grow on sucrose in the presence of 2-deoxyglucose. We identified recessive mutations in three major complementation groups that cause constitutive (glucose-insensitive) secreted invertase synthesis. Two groups comprise alleles of the previously identified HXK2 and REG1 genes, and the third group was designated cid1 (constitutive invertase derepression). The effect of cid1 on SUC2 expression is mediated by the SUC2 upstream regulatory region, as judged by the constitutive expression of a SUC2-LEU2-lacZ fusion in which the LEU2 promoter is under control of SUC2 upstream sequences. A cid1 mutation also causes glucose-insensitive expression of maltase. The previously isolated constitutive mutation ssn6 is epistatic to cid1, reg1 and hxk2 for very high level constitutive invertase expression. Mutations in SNF genes that prevent derepression of invertase are epistatic to cid1, reg1 and hxk2; we have previously shown that ssn6 has different epistasis relationships with snf mutations. The constitutive mutation tup1 was found to resemble ssn6 in its genetic interactions with snf mutations. These findings suggest that CID1, REG1 and HXK2 are functionally distinct from SSN6 and TUP1.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Cell Biol. 1983 Mar;3(3):439-47 - PubMed
    1. Mol Cell Biol. 1986 Nov;6(11):3569-74 - PubMed
    1. Mol Cell Biol. 1985 Nov;5(11):3035-40 - PubMed
    1. Methods Enzymol. 1975;42:504-11 - PubMed
    1. Biochim Biophys Acta. 1966 Jun 15;118(3):522-37 - PubMed

Publication types

Substances