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. 2022 Apr 12;9(1):161.
doi: 10.1038/s41597-022-01266-w.

A multi-million image Serial Femtosecond Crystallography dataset collected at the European XFEL

Henry J Kirkwood #  1 , Raphael de Wijn #  2 , Grant Mills #  2 , Romain Letrun  2 , Marco Kloos  2 , Mohammad Vakili  2 , Mikhail Karnevskiy  2 , Karim Ahmed  2 , Richard J Bean  2 , Johan Bielecki  2 , Fabio Dall'Antonia  2 , Yoonhee Kim  2 , Chan Kim  2 , Jayanath Koliyadu  2 , Adam Round  2   3 , Tokushi Sato  2 , Marcin Sikorski  2 , Patrik Vagovič  2 , Jolanta Sztuk-Dambietz  2 , Adrian P Mancuso  2   4
Affiliations

A multi-million image Serial Femtosecond Crystallography dataset collected at the European XFEL

Henry J Kirkwood et al. Sci Data. .

Abstract

Serial femtosecond crystallography is a rapidly developing method for determining the structure of biomolecules for samples which have proven challenging with conventional X-ray crystallography, such as for membrane proteins and microcrystals, or for time-resolved studies. The European XFEL, the first high repetition rate hard X-ray free electron laser, provides the ability to record diffraction data at more than an order of magnitude faster than previously achievable, putting increased demand on sample delivery and data processing. This work describes a publicly available serial femtosecond crystallography dataset collected at the SPB/SFX instrument at the European XFEL. This dataset contains information suitable for algorithmic development for detector calibration, image classification and structure determination, as well as testing and training for future users of the European XFEL and other XFELs.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Example of single crystal diffraction data measured by AGIPD (left). Off-axis microscope for monitoring the overlap of the liquid jet and X-ray beam (right). The image was acquired with a single 800 nm wavelength, 65 fs duration laser pulse from the SASE1 pump-probe laser system, 110 ns after the first X-ray pulse in the train.
See this image and copyright information in PMC

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