Drosophila Model for Studying Gut Microbiota in Behaviors and Neurodegenerative Diseases

doi:10.3390/biomedicines10030596

Review

. 2022 Mar 3;10(3):596.

doi: 10.3390/biomedicines10030596.

Drosophila Model for Studying Gut Microbiota in Behaviors and Neurodegenerative Diseases

Meng-Hsuan Chiang¹, Shuk-Man Ho¹, Hui-Yu Wu¹, Yu-Chun Lin¹, Wan-Hua Tsai², Tony Wu^{3

4

5}, Chih-Ho Lai^{1

3

6

7}, Chia-Lin Wu^{1

3

8}

Affiliations

¹ Graduate Institute of Biomedical Sciences, Department of Biochemistry, Department of Microbiology, Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.
² Research and Development Department, GenMont Biotech Incorporation, Tainan 74144, Taiwan.
³ Department of Neurology, Molecular Infectious Disease Research Center, Department of Pediatrics, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.
⁴ Department of Neurology, New Taipei Municipal Tucheng Hospital, Tucheng 23652, Taiwan.
⁵ Department of Neurology, Xiamen Chang Gung Hospital, Xiamen 361028, China.
⁶ Department of Medical Research, Graduate Institute of Biomedical Sciences, China Medical University and Hospital, Taichung 40402, Taiwan.
⁷ Department of Nursing, Asia University, Taichung 41354, Taiwan.
⁸ Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan.

PMID: 35327401
PMCID: PMC8945323
DOI: 10.3390/biomedicines10030596

Review

Drosophila Model for Studying Gut Microbiota in Behaviors and Neurodegenerative Diseases

Meng-Hsuan Chiang et al. Biomedicines. 2022.

. 2022 Mar 3;10(3):596.

doi: 10.3390/biomedicines10030596.

Authors

Meng-Hsuan Chiang¹, Shuk-Man Ho¹, Hui-Yu Wu¹, Yu-Chun Lin¹, Wan-Hua Tsai², Tony Wu^{3

4

5}, Chih-Ho Lai^{1

3

6

7}, Chia-Lin Wu^{1

3

8}

Affiliations

¹ Graduate Institute of Biomedical Sciences, Department of Biochemistry, Department of Microbiology, Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.
² Research and Development Department, GenMont Biotech Incorporation, Tainan 74144, Taiwan.
³ Department of Neurology, Molecular Infectious Disease Research Center, Department of Pediatrics, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.
⁴ Department of Neurology, New Taipei Municipal Tucheng Hospital, Tucheng 23652, Taiwan.
⁵ Department of Neurology, Xiamen Chang Gung Hospital, Xiamen 361028, China.
⁶ Department of Medical Research, Graduate Institute of Biomedical Sciences, China Medical University and Hospital, Taichung 40402, Taiwan.
⁷ Department of Nursing, Asia University, Taichung 41354, Taiwan.
⁸ Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan.

PMID: 35327401
PMCID: PMC8945323
DOI: 10.3390/biomedicines10030596

Abstract

Mounting evidence indicates that the gut microbiota is linked to several physiological processes and disease development in mammals; however, the underlying mechanisms remained unexplored mostly due to the complexity of the mammalian gut microbiome. The fruit fly, Drosophila melanogaster, is a valuable animal model for studying host-gut microbiota interactions in translational aspects. The availability of powerful genetic tools and resources in Drosophila allowed the scientists to unravel the mechanisms by which the gut microbes affect fitness, health, and behavior of their hosts. Drosophila models have been extensively used not only to study animal behaviors (i.e., courtship, aggression, sleep, and learning & memory), but also some human related neurodegenerative diseases (i.e., Alzheimer's disease and Parkinson's disease) in the past. This review comprehensively summarizes the current understanding of the gut microbiota of Drosophila and its impact on fly behavior, physiology, and neurodegenerative diseases.

Keywords: Drosophila melanogaster; behaviors; gut−brain axis; learning and memory; microbiota; neurodegenerative diseases.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

**Figure 1**
Comparison of human and *Drosophila* digestive tracts. (a) Digestive tracts of humans and *Drosophila*. The functional regions of the digestive tract in *Drosophila* and humans are similar and include esophagus, anterior and posterior midgut (small intestine), large intestine (hindgut), and stomach (crop). (b) Subregions of the *Drosophila* digestive tract depicting the foregut, midgut, and hindgut. Foregut contains crop and proventriculus. The midgut is further divided into R1-R5 regions. (c) GFP-tagged *Lactobacillus* was fed to adult *Drosophila* and confocal images show the distribution of *Lactobacillus* (green) in different regions of the digestive tract. The magnified images of crop, R2, R4, and R5 regions are showed in c1 to c4. The sample was immunostained with anti-integrin-βPS antibody (red). Scale bar, 500 μm.

See this image and copyright information in PMC

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References

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[1] Konopka R.J., Benzer S. Clock mutants of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA. 1971;68:2112–2116. doi: 10.1073/pnas.68.9.2112. - DOI - PMC - PubMed

[2] Konopka R.J., Benzer S. Clock mutants of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA. 1971;68:2112–2116. doi: 10.1073/pnas.68.9.2112. - DOI - PMC - PubMed

[3] Dow M.A., Schilcher F.V. Aggression and mating success in Drosophila melanogaster. Nature. 1975;254:511–512. doi: 10.1038/254511a0. - DOI - PubMed

[4] Dow M.A., Schilcher F.V. Aggression and mating success in Drosophila melanogaster. Nature. 1975;254:511–512. doi: 10.1038/254511a0. - DOI - PubMed

[5] Hall J.C. The mating of a fly. Science. 1994;264:1702–1714. doi: 10.1126/science.8209251. - DOI - PubMed

[6] Hall J.C. The mating of a fly. Science. 1994;264:1702–1714. doi: 10.1126/science.8209251. - DOI - PubMed

[7] Tempel B.L., Bonini N., Dawson D.R., Quinn W.G. Reward learning in normal and mutant Drosophila. Proc. Natl. Acad. Sci. USA. 1983;80:1482–1486. doi: 10.1073/pnas.80.5.1482. - DOI - PMC - PubMed

[8] Tempel B.L., Bonini N., Dawson D.R., Quinn W.G. Reward learning in normal and mutant Drosophila. Proc. Natl. Acad. Sci. USA. 1983;80:1482–1486. doi: 10.1073/pnas.80.5.1482. - DOI - PMC - PubMed

[9] Tully T., Quinn W.G. Classical conditioning and retention in normal and mutant Drosophila melanogaster. J. Comp. Physiol. A. 1985;157:263–277. doi: 10.1007/BF01350033. - DOI - PubMed

[10] Tully T., Quinn W.G. Classical conditioning and retention in normal and mutant Drosophila melanogaster. J. Comp. Physiol. A. 1985;157:263–277. doi: 10.1007/BF01350033. - DOI - PubMed

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Drosophila Model for Studying Gut Microbiota in Behaviors and Neurodegenerative Diseases

Affiliations

Drosophila Model for Studying Gut Microbiota in Behaviors and Neurodegenerative Diseases

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

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Cited by

References

Publication types

Grants and funding

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Miscellaneous

Abstract

Conflict of interest statement

Figures

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References

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