Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Apr-Jun;13(2):100542.
doi: 10.1016/j.jaim.2022.100542. Epub 2022 Feb 25.

Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study

Somnath D Bhinge  1 , Namdeo R Jadhav  2 , Dheeraj S Randive  3 , Mangesh A Bhutkar  3 , Rohankumar Chavan  4 , Bajarang V Kumbhar  5
Affiliations

Isolation and identification of hair growth potential fraction from active plant extract of Blumea eriantha DC grown in Western Ghat of India: In silico study

Somnath D Bhinge et al. J Ayurveda Integr Med. 2022 Apr-Jun.

Abstract

Background: In Aayurveda, Blumea eriantha DC has been used in the management of various diseases and is found to exhibit antioxidant and anti-hyperlipidemic, hypoglycemic, anti-diarrhoeal, larvicidal, antimicrobial properties.

Objective: The present study has focused on isolation of the active fraction from B. eriantha DC extract and to investigate its effect as a hair growth promoter along with identification of phytoconstituent(s) responsible for hair growth activity and its probable mechanism of action.

Materials and methods: Our work introduces an effective isolation protocol for the active fraction from B. eriantha DC extract using chromatographic techniques. Fraction A was isolated by using mobile phase toluene:acetone (9:1). In-vitro and in-vivo methods were executed for the evaluation of hair growth activity. Moreover, the docked conformations of the isolated phytoconstituent Dimethyl sulfone was compared to Minoxidil for selected proteins namely 2FGF, 2PVC and 4U7P. The PDB identifications 2PVC (DNMT3L recognizes unmethylated histone H3 lysine 4), 4U7P (Crystal structure of DNMT3A-DNMT3L complex and 2FGF (Human Basic Fibroblast Growth Factor) were downloaded from Protein Data Bank.

Results: The study data revealed that B. eriantha DC alcoholic extracts exhibited prominent hair growth activity and it was affirmed that Dimethyl sulfone a phyto-constituent isolated from B. eriantha DC alcoholic extract contributed for the same.

Conclusion: The findings strongly suggest hair growth promotion potential of the extract of B. eriantha DC.

Keywords: Aayurveda; Blumea eriantha DC; GC/MS; HPLC; Hair growth; Molecular docking.

PubMed Disclaimer

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
GC-MS Chromatogram and TLC of Fraction A, UV, IR and NMR and HPLC graph of Dimethyl sulfone.
Fig. 2
Fig. 2
Hair growth pattern after 30th day effect.
Fig. 3
Fig. 3
Effect of BE ethanolic extract on qualitative hair growth parameter (A). Histopathological studies, the number of hair follicle in subcutis layer of animal treated with 3% BE alcoholic extract, 1.5% BE alcoholic extract, 1% Minoxidil solution and control vehicle (B) (The results are shown as the mean values ± SEM, ∗∗P < 0.0, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, when compared to respective control values by Student’s t-test).
Fig. 4
Fig. 4
2D & 3D binding mode of the Minoxidil and Dimethyl sulfone on 2FGF, 2PVC and 4U7P. Where, in 2D images, the hydrogen bond and Pi-sulfur are represented in fluorescent green and orange yellow in color respectively. And, In 3D image, the Helix, Turn/C Alpha and Sheet are represented in yellow, green and blue in color respectively.
Supplemental 2
Supplemental 2
Diagrammatic representation of present research work.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Stough D., Stenn K., Haber R. Psychological effect, pathophysiology and management of androgenetic alopecia in men. Mayo Clin Proc. 2005;80:1316–1322. - PubMed
    1. Savali A., Bhinge S., Chittapurkar H. Evaluation of hair growth promoting activity of Musa Paradisiaca unripe fruit extract. J. Nat. Pharmaceuticals. 2011;2(3):120–124.
    1. Suraj R., Rejitha G., Sunilson J.A., Anandarajgopal K., Promwichit P. In vivo hair growth activity of Prunus dulcis seeds in rats. Biol Med. 2009;4:34–38.
    1. Randive D.S., Bhinge S.D., Sayyad S.F., Wadkar G.H. Comparative standardization of marketed formulations of fermented biomedicine – arjunaristha. Indones J Pharm. 2016;27(4):220–225.
    1. Bhinge S.D., Bhutkar M.A., Randive D.S., Wadkar G.H., Todkar S.S. Development and evaluation of antimicrobial polyherbal gel. Ann Pharm Fr. 2017;75:349–358. - PubMed