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. 2022 Feb 6;14(2):331.
doi: 10.3390/v14020331.

Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea

Yanfen Wang  1 , Hang Zhao  1 , Jiayuan Cao  1 , Xinming Yin  1 , Yashuang Guo  1 , Lihua Guo  2 , Haiyan Wu  3 , Meng Zhang  1   2
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Characterization of a Novel Mycovirus from the Phytopathogenic Fungus Botryosphaeria dothidea

Yanfen Wang et al. Viruses. .

Abstract

Botryosphaeria dothidea is, globally, one of the most economically important phytopathogenic fungi worldwide, causing the canker and dieback of fruit trees. An increasing number of viruses infecting B. dothidea have lately been reported, several of which could confer hypovirulence. In this study, isolated from strain ZM170285-1 of B. dothidea, a novel double-stranded RNA (dsRNA) mycovirus, tentatively named Botryosphaeria dothidea partitivirus 2 (BdPV2), was identified well. The BdPV2 harbored three dsRNA segments (1-3) with lengths of 1751, 1568, and 1198 bp, which encoded an RNA-dependent RNA polymerase (RdRp), a capsid protein (CP), and a hypothetical protein of unknown function, respectively. BLASTp searches revealed that the predicted protein sequences of dsRNA1 and dsRNA2 had the highest identities (74.95% and 61.01%) with the corresponding dsRNAs of Penicillium stoloniferum virus S (PsV-S), whereas dsRNA3 shared the highest identity (32.95%) with the dsRNA3 of Aspergillus ochraceous virus 1 (AoV1). Phylogenetic analysis indicated that BdPV2 belonged to the Gammapartitivirus genus and Partitiviridae family. To our knowledge, this is the first report of a Gammapartitivirus in B. dothidea.

Keywords: Botryosphaeria dothidea; Botryosphaeria dothidea partitivirus 2 (BdPV2); Gammapartitivirus.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Genomic organization of BdPV2. (A) Lane M, DL5000 DNA marker; Lane N, dsRNA extracted from strain ZM170285-1; Lanes D and S1, dsRNA was treated with DNase I and S1 nuclease. (B) Schematic representation of genomic organization of BdPV2. (C) Multiple sequence alignment of 5′- and 3′-UTRs of BdPV2. *, conserved amino acid residues.
Figure 2
Figure 2
Phylogenetic analysis of BdPV2. Maximum-likelihood (ML) phylogenetic analysis based on amino acid sequences of (A) RdRp and (B) CP of BdPV2 and other selected members of seven genera of the Partitiviridae family. Phylogenetic tree was generated using MEGA 7 with 1000 bootstrap replicates. Triangle indicates position of BdPV2. (C) Multiple alignments of amino acid sequences of RdRp motifs of BdPV2 and other selected mycoviruses in Partitiviridae family. Six motifs (III–VIII) detected in conserved RdRp sequence. Similar amino acid residues are shaded in black.
Figure 3
Figure 3
Biological characteristic comparison between strains ZM170285-1 and ZM180088. (A) Colony morphology and growth rates of strains ZM170285-1 and ZM180088 grown at 25 °C for 3 days on PDA medium. (B) Pathogenicity assay and lesion diameters of two strains grown on detached walnut stems at 10 days post inoculation at 30 °C, and approximately 90% relative humidity. **, indicates a significant difference (p < 0.01) between strains ZM170285-1 and ZM180088.
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