iTRAQ and two-dimensional-LC-MS/MS reveal NAA10 is a potential biomarker in esophageal squamous cell carcinoma

doi:10.1002/prca.202100081

. 2022 May;16(3):e2100081.

doi: 10.1002/prca.202100081. Epub 2022 Feb 25.

iTRAQ and two-dimensional-LC-MS/MS reveal NAA10 is a potential biomarker in esophageal squamous cell carcinoma

Dong Wang¹, Jinyan Chen^{2

3}, Junyong Han^{2

3}, Kun Wang^{2

3}, Weimin Fang⁴, Jingjun Jin^{2

3}, Shijie Xue^{2

3}

Affiliations

¹ Department of General Surgery (Emergency Surgery), Fujian Medical University Union Hospital, Fujian, China.
² Institute for Immunology, Fujian Academy of Medical Sciences, Fuzhou, Fujian, China.
³ Fujian Provincial Key Laboratory of Medical Analysis, Fuzhou, Fujian, China.
⁴ Fujian Provincial Cancer Hospital, Fuzhou, Fujian, China.

PMID: 35182098
DOI: 10.1002/prca.202100081

iTRAQ and two-dimensional-LC-MS/MS reveal NAA10 is a potential biomarker in esophageal squamous cell carcinoma

Dong Wang et al. Proteomics Clin Appl. 2022 May.

. 2022 May;16(3):e2100081.

doi: 10.1002/prca.202100081. Epub 2022 Feb 25.

Authors

Dong Wang¹, Jinyan Chen^{2

3}, Junyong Han^{2

3}, Kun Wang^{2

3}, Weimin Fang⁴, Jingjun Jin^{2

3}, Shijie Xue^{2

3}

Affiliations

¹ Department of General Surgery (Emergency Surgery), Fujian Medical University Union Hospital, Fujian, China.
² Institute for Immunology, Fujian Academy of Medical Sciences, Fuzhou, Fujian, China.
³ Fujian Provincial Key Laboratory of Medical Analysis, Fuzhou, Fujian, China.
⁴ Fujian Provincial Cancer Hospital, Fuzhou, Fujian, China.

PMID: 35182098
DOI: 10.1002/prca.202100081

Abstract

Purpose: Esophageal squamous cell carcinoma (ESCC) is one of the most common and serious malignancies in China. However, the exact mechanisms of tumor progression are still unclear. Thus, identifying biomarkers for early diagnosis, prognostic and recurrence assessment of ESCC is necessary.

Experimental design: iTRAQ was used to identify differentially expressed proteins (DEPs) in tumor tissues. N-alpha-acetyltransferase 10 (NAA10) is confirmed and validated by immunohistochemistry and western blotting. Furthermore, the effects of NAA10 on TE-1 cells were detected by CCK-8, colonies formation, anchorage-independent growth in soft agar, migration and transwell assays. LinkedOmics was used to identify differential gene expression with NAA10 and to analyze Gene Ontology and KEGG pathways. Coexpression gene network was conducted by the STRING database and Cytoscape software (MCODE plug-in).

Results: 516 DEPs were identified. NAA10 was downregulated in cancer tissues and selected for further confirmed. Furthermore, NAA10 can inhibit proliferation and tumorigenesis, and suppress migration and invasion of TE-1. Functional network analysis suggested that NAA10 regulates the ribosome pathways involving eight ribosomal proteins.

Conclusion and clinical relevance: These findings clearly demonstrated that NAA10 is a tumor suppressor and novel potential biomarker for ESCC, laying a foundation for further study of the role of NAA10 in carcinogenesis.

Keywords: NAA10; biomarker; esophageal squamous cell carcinoma.

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References

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[1] Soerjomataram, I., Lortet-Tieulent, J., Parkin, D. M., Ferlay, J., Mathers, C., Forman, D., & Bray, F. (2012). Global burden of cancer in 2008: A systematic analysis of disability-adjusted life-years in 12 world regions. Lancet, 380, 1840-1850.

[2] Soerjomataram, I., Lortet-Tieulent, J., Parkin, D. M., Ferlay, J., Mathers, C., Forman, D., & Bray, F. (2012). Global burden of cancer in 2008: A systematic analysis of disability-adjusted life-years in 12 world regions. Lancet, 380, 1840-1850.

[3] Chen, W., Zheng, R., Baade, P. D., Zhang, S., Zeng, H., Bray, F., Jemal, A., Yu, X. Q., & He, J. (2016). Cancer statistics in China, 2015. CA: A Cancer Journal for Clinicians, 66, 115-132.

[4] Chen, W., Zheng, R., Baade, P. D., Zhang, S., Zeng, H., Bray, F., Jemal, A., Yu, X. Q., & He, J. (2016). Cancer statistics in China, 2015. CA: A Cancer Journal for Clinicians, 66, 115-132.

[5] Shinsuke, T., Tsuyoshi, N., Yoshiaki, T., Shoichi, F., Tomotaka, S., & Katsunobu, K. (2007). Assessment of clinical outcome in patients with esophageal squamous cell carcinoma using TNM classification score and molecular biological classification. Annals of Surgical Oncology, 14(4), 1431-1438.

[6] Shinsuke, T., Tsuyoshi, N., Yoshiaki, T., Shoichi, F., Tomotaka, S., & Katsunobu, K. (2007). Assessment of clinical outcome in patients with esophageal squamous cell carcinoma using TNM classification score and molecular biological classification. Annals of Surgical Oncology, 14(4), 1431-1438.

[7] Wei, W. Q., Chen, Z. F., He, Y.-T., Feng, H., Hou, J., Lin, D.- M., Li, X. Q., Guo, C. L., Li, S. S., Wang, G.-Q., Dong, Z. W., Abnet, C. C., & Qiao, Y. L. (2015). Long-term follow-up of a community assignment, one-time endoscopic screening study of esophageal cancer in China. Journal of Clinical Oncology, 33, 1951-1957.

[8] Wei, W. Q., Chen, Z. F., He, Y.-T., Feng, H., Hou, J., Lin, D.- M., Li, X. Q., Guo, C. L., Li, S. S., Wang, G.-Q., Dong, Z. W., Abnet, C. C., & Qiao, Y. L. (2015). Long-term follow-up of a community assignment, one-time endoscopic screening study of esophageal cancer in China. Journal of Clinical Oncology, 33, 1951-1957.

[9] Pawar, H., Kashyap, M. K., Sahasrabuddhe, N. A., Renuse, S., Harsha, H. C., Kumar, P., Sharma, J., Kandasamy, K., Marimuthu, A., Nair, B., Rajagopalan, S., Maharudraiah, J., Premalatha, C. S., Kumar, K. V. V., Vijayakumar, M., Chaerkady, R., Prasad, T. S. K., & Kumar, R. V., & Pandey, A. (2011). Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discovery. Cancer Biology & Therapy, 12, 510-522.

[10] Pawar, H., Kashyap, M. K., Sahasrabuddhe, N. A., Renuse, S., Harsha, H. C., Kumar, P., Sharma, J., Kandasamy, K., Marimuthu, A., Nair, B., Rajagopalan, S., Maharudraiah, J., Premalatha, C. S., Kumar, K. V. V., Vijayakumar, M., Chaerkady, R., Prasad, T. S. K., & Kumar, R. V., & Pandey, A. (2011). Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discovery. Cancer Biology & Therapy, 12, 510-522.

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iTRAQ and two-dimensional-LC-MS/MS reveal NAA10 is a potential biomarker in esophageal squamous cell carcinoma

Affiliations

iTRAQ and two-dimensional-LC-MS/MS reveal NAA10 is a potential biomarker in esophageal squamous cell carcinoma

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