Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Apr;166(1):205-11.
doi: 10.1128/jb.166.1.205-211.1986.

Nucleotide pool in pho regulon mutants and alkaline phosphatase synthesis in Escherichia coli

N N Rao, E Wang, J Yashphe, A Torriani

Nucleotide pool in pho regulon mutants and alkaline phosphatase synthesis in Escherichia coli

N N Rao et al. J Bacteriol. 1986 Apr.

Abstract

The intracellular nucleotide pool of Escherichia coli W3110 reproducibly changes from conditions of growth in phosphate excess to phosphate starvation, with at least two nucleotides appearing under starvation conditions and two nucleotides appearing only under excess phosphate conditions. Strains bearing a deletion of the phoA gene show the same pattern, indicating that dephosphorylation by alkaline phosphatase is not responsible for the changes. Strains with mutations in the phoU gene, which result in constitutive expression of the pho regulon, show the nucleotide pattern of phosphate-starved cells even during phosphate excess growth. These changes in nucleotides are therefore due to phoU mutation but not to alkaline phosphatase constitutivity. In fact, a phoR (phoR68) mutant strain has the patterns of the wild type in spite of being constitutive for alkaline phosphatase. That these nucleotides might be specific signals for pho regulon expression was supported by the fact that the two nucleotides appearing under phosphate starvation induced the synthesis of alkaline phosphatase in repressed permeabilized wild-type cells under conditions of phosphate excess.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochim Biophys Acta. 1960 Mar 11;38:460-9 - PubMed
    1. J Bacteriol. 1968 Oct;96(4):1200-7 - PubMed
    1. J Bacteriol. 1972 May;110(2):616-23 - PubMed
    1. Bacteriol Rev. 1972 Dec;36(4):525-57 - PubMed
    1. J Bacteriol. 1974 Sep;119(3):736-47 - PubMed

Publication types

LinkOut - more resources